碱性成纤维细胞生长因子在快速牙移动中的表达变化  

Expression of basic fibroblast growth factor in rapid tooth movement

作　　者：杨乐乐 孙枭 陈曦[3] 李佩 Yang Lele;Sun Xiao;Chen Xi;Li Pei(Key laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,Xi'an Jiaotong University,Xi'an 710004,China;Department of Pediatric Dentistry,College of Stomatology,Xi'an Jiaotong University,Xi'an 710004,China;Department of Stomatology,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China;Department of Stomatology,Xi'an No.9 Hospital,Xi'an 710054,China)

机构地区：[1]西安交通大学口腔医院陕西省颅颌面精准医学研究重点实验室,陕西西安710004 [2]西安交通大学口腔医院儿童口腔科,陕西西安710004 [3]西安交通大学第一附属医院口腔科,陕西西安710061 [4]西安市第九医院口腔科,陕西西安710054

出　　处：《兰州大学学报（医学版）》2024年第11期17-23,共7页Journal of Lanzhou University（Medical Sciences）

基　　金：陕西省重点研发计划资助项目(2018SF-037)。

摘　　要：目的探讨减阻牵张快速牙移动过程中碱性成纤维细胞生长因子(bFGF)与移动牙张力侧牙周组织改建的相关性。方法12只Beagle犬按加力5、10、15 d和加力15 d保持90 d分为4组,每组3只。组内每犬下颌左、右侧随机采用减阻牵张或常规正畸方法。拔除下颌第二前磨牙,以第三前磨牙(支抗牙)为支抗移动第一前磨牙(移动牙)。减阻牵张组行牙槽间隔减阻术和2次/d牵张力。各时间点分别测量移动牙及支抗牙的移动距离;苏木精-伊红染色观察移动牙张力侧牙周组织的改建情况及毛细血管密度的变化;免疫组织化学染色观察增殖细胞核抗原及bFGF的平均光密度变化。结果各加力阶段,减阻牵张组较常规组移动牙的移动距离显著增加(P<0.05),支抗牙移动距离差异无统计学意义(P>0.05);苏木精-伊红染色显示减阻牵张组张力侧牙周组织改建更活跃;各加力阶段,减阻牵张组张力侧牙周膜内的bFGF表达均高于常规组(P<0.05),加力15 d保持90 d时差异无统计学意义(P>0.05);移动牙张力侧牙周膜内毛细血管密度及增殖细胞核抗原阳性表达变化趋势与bFGF基本一致。结论减阻牵张方法引发的牙周组织快速改建可能与bFGF促进血管增殖、促进细胞增殖分化和促进骨组织改建等生物学机制相关。Objective To investigate the correlation between basic fibroblast growth factor(bFGF)and the reconstruction of periodontal tissue on the tension side of the moving tooth during rapid tooth movement due to a reduction of resistance and distraction.Methods Twelve beagle dogs were divided into four groups according to the distraction for 5 days,10 days,15 days,retaining 90 days after distraction for 15 days,with three dogs in each group.The left and right sides in mandibular of each dog were randomly treated with two different procedures:reducing resistance and distraction,or conventional orthodontics.The second premolar of the mandible was extracted,and the third premolar(anchorage tooth)was used as an anchorage to move the first premolar(moving tooth).The reducing resistance and distraction group underwent alveolar septal reduction and distracted twice a day.The moving distance of the moving tooth and the anchorage tooth was measured at various time points.Hematoxylin-eosin staining was used to observe the remodeling of the periodontal tissue and change in capillary density on the tension side of the moving tooth.Immunohisto-chemical staining was used to observe the changes in the average optical density of proliferating cell nuclear antigen and bFGF.Results During each stage of distraction,the moving distance of the moving tooth in the reducing resistance and distraction group was significantly increased compared to the conventional group(P<0.05),while there was no significant difference in the moving distance of the anchorage tooth(P>0.05).Hematoxylin-eosin staining showed that the periodontal tissue on the tension side of the resistance and distraction group was remodeling more actively.During each stage of force application,the expression of bFGF in the periodontal tissue on the tension side of the reducing tension group was higher than that in the conventional group(P<0.05),and there was no significant difference at 90 days(P>0.05).The trend of changes in capillary density and positive expression of the proli

关 键 词：减阻牵张 牙周组织快速改建 毛细血管密度 增殖细胞核抗原 碱性成纤维细胞生长因子 

分 类 号：R783.5[医药卫生—口腔医学]