沉默STAT3对姜黄素抑制食管癌细胞增殖、迁移的影响  

Effect of Silence of STAT3 on Proliferation and Migration of Esophageal Cancer Cell Treated by Curcumin

作　　者：王霞[1] 叶曼红[1] 黄智璇[1] 杜敏[1] 黄海潮[1] 张娜 徐单单 WANG Xia;YE Manhong;HUANG Zhixuan(School of Health Management and Biotechnology,Food and Drug Vocational College of Guangdong,Guangzhou City,Guangdong Province 510520)

机构地区：[1]广东食品药品职业学院健康管理与生物技术学院,广东省广州市510520

出　　处：《医学理论与实践》2024年第24期4141-4144,4170,共5页The Journal of Medical Theory and Practice

基　　金：广东省教育厅科研项目(2022KTSCX270);广东省中医药局项目(20231248);广东省医学科学技术研究基金项目(A2024075);广东食品药品职业学院校级项目(2022ZR03)。

摘　　要：目的:探讨STAT3沉默与姜黄素(curcumin)联用对食管癌细胞增殖、侵袭的影响。方法:食管癌临床标本及癌旁组织收集于中山大学肿瘤防治中心,免疫组化实验用于研究肿瘤组织与癌旁组织的STAT3表达水平;构建沉默STAT3的慢病毒干扰载体shSTAT3与阴性对照载体shNC并使用转染试剂Lipofectamine^(TM)3000转染食管癌细胞。Western blot与qRT-PCR实验检测沉默STAT3后其表达水平;MTT、克隆形成与划痕试验评价姜黄素对细胞的增殖、迁移能力。Western blot实验检测凋亡家族蛋白水平变化。结果:qRT-PCR与免疫组织化学研究结果表明,与癌旁组织相比,STAT3在食管癌组织标本的表达水平更高。与对照组相比,沉默STAT3后,食管癌细胞受到姜黄素作用,其增殖活性显著下降,克隆形成与迁移能力明显降低(P<0.05),凋亡家族蛋白变化显著。结论:STAT3的沉默与姜黄素联用降低了食管癌细胞增殖、迁移。沉默STAT3有效帮助姜黄素抑制食管癌细胞增殖、迁移能力。Objective:To investigate the effect of silencing STAT3 and curcumin on the proliferation and migration of the esophageal cancer cell.Methods:Esophageal cancer clinical samples were collected by Sun Yat-sen University Cancer Center.The STAT3 expression level in Esophageal cancer clinical samples was analyzed by immunohistochemistry study.The shSTAT3 lentiviral vector and negative control vector were designed and constructed.Esophageal cancer cells were transfected by the lentiviral vectors using Lipofectamine transfection reagent^(TM)3000.The protein and mRNA expression levels of STAT3 in esophageal cancer cell were detected by Western blot and qRT-PCR assays.The inhibitory effect of shSTAT3 and curcumin on the proliferation activity was evaluated by MTT assay.The colony formation,western blot and scratch assays were used to test the anti-tumor ability of curcumin.The apoptosis proteins were analyzed by Western blot assay.Results:The level of STAT3 in tumor tissue was higher compred with the adjacent tissue demonstrated by qRT-PCR and immunohistochemistry study.Compared with the control group,silencing STAT3 with curcumin significantly reduced the proliferation ability and cell migration of esophageal cancer cell(P<0.05).The apoptosis proteins demonstrated significant changes.Conclusion:STAT3 silence with curcumin inhibits the proliferation,migration of esophageal cancer cell.Silence of STAT3 enhances curcumin-induced proliferation and migration of esophageal cancer cell.

关 键 词：信号转导子与转录激活子3 姜黄素 增殖 迁移 食管癌细胞 

分 类 号：R735.1[医药卫生—肿瘤]