CCN1对食管癌细胞APRIL/BAFF表达的影响  

Effect of CCN1 on APRIL/BAFF expression in esophageal cancer cells

作　　者：邢玲玲 刘琳[2] 江振宇[2] 柴建原 武金宝[2] XING Lingling;LIU Lin;JIANG Zhenyu;CHAI Jianyuan;WU Jinbao(Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040,China;Inner Mongolia Digestive Disease Research Institute,the Second Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology)

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014040 [2]内蒙古科技大学包头医学院第二附属医院内蒙古消化病研究所

出　　处：《包头医学院学报》2024年第12期13-17,共5页Journal of Baotou Medical College

基　　金：内蒙古自然科学基金项目(2020LH08005);内蒙古自治区高等学校科学研究项目(JZY22057,NJZY22095);包头医学院青年科技人才发展计划(YTT-QNGG2022065);内蒙古医学科学院临床需求性基础研究项目(2023GLLH0213);内蒙古自治区教育科学研究“十四五”规划课题(NGJGH2023539)。

摘　　要：目的:探究CCN1蛋白在食管癌中对增殖诱导配体(APRIL)和B细胞刺激因子(BAFF)所起到的作用。方法:用质粒转染技术在细胞株KYSE150及OE-19中过表达CCN1,Western blot验证其转染效果,同时检测APRIL和BAFF的表达情况。结果:在KYSE150细胞中过表达CCN1后,APRIL/BAFF高表达(P<0.05);在OE19细胞中过表达CCN1后,APRIL/BAFF低表达(P<0.05)。结论:CCN1在食管鳞癌细胞KYSE150中对APRIL/BAFF为激活作用,在食管腺癌细胞OE19中对APRIL/BAFF为抑制作用。Objective:To investigate the role of CCN1 protein in a proliferation-inducing ligand(APRIL)and B-cell activating factor(BAFF)in esophageal cancer.Methods:CCN1 was overexpressed in KYSE150 and OE-19 cell lines by plasmid transfection technique.The transfection effect was verified by Western blot,and the expression of APRIL and BAFF was detected.Results:APRIL/BAFF was highly expressed after overexpression of CCN1 in KYSE150 cells(P<0.05),and APRIL/BAFF was lowly expressed after overexpression of CCN1 in OE19 cells(P<0.05).Conclusion:CCN1 activates APRIL/BAFF in esophageal squamous cell carcinoma cell line KYSE150 and inhibits APRIL/BAFF in esophageal adenocarcinoma cell line OE19.

关 键 词：CCN1 增殖诱导配体 细胞刺激因子 食管癌 

分 类 号：R735.1[医药卫生—肿瘤]