Correlations of the expression of Cx43,SCF^(FBXW7),p-cyclin E1(Ser73),p-cyclin E1(Thr77)and p-cyclin E1(Thr395)in colon cancer tissues  

作　　者：Rong-Gang Luan Ming-Da Liu Zi-Feng Deng Cong-Lan Lu Mei-Ling Yu Ming-Yu Zhang Rong Liu Ran An You-Liang Yao Dong-Bei Guo Yong-Xing Zhang Lei Zhao 

机构地区：[1]Department of Gastrointestinal Surgery,Air Force Hospital of Eastern Theater,Anhui Medical University,Nanjing 230032,Jiangsu Province,China [2]State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,School of Public Health,Xiamen University,Xiamen 361102,Fujian Province,China [3]Trauma Surgery,Air Force Hospital of Eastern Theater,Anhui Medical University,Nanjing 230032,Jiangsu Province,China [4]Department of Orthopaedics,Air Force Hospital of Eastern Theater,Anhui Medical University,Nanjing 230032,Jiangsu Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2025年第1期207-213,共7页世界胃肠肿瘤学杂志（英文）

基　　金：Supported by Innovative Practice Platform for Undergraduate Students,School of Public Health Xiamen University,No.2021001.

摘　　要：BACKGROUND Previous cellular studies have demonstrated that elevated expression of Cx43 promotes the degradation of cyclin E1 and inhibits cell proliferation through ubiquitination.Conversely,reduced expression results in a loss of this capacity to facilitate cyclin E degradation.The ubiquitination and degradation of cyclin E1 may be associated with phosphorylation at specific sites on the protein,with Cx43 potentially enhancing this process by facilitating the phosphorylation of these critical residues.AIM To investigate the correlation between expression of Cx43,SKP1/Cullin1/F-box(SCF)FBXW7,p-cyclin E1(ser73,thr77,thr395)and clinicopathological indexes in colon cancer.METHODS Expression levels of Cx43,SCF^(FBXW7),p-cyclin E1(ser73,thr77,thr395)in 38 clinical colon cancer samples were detected by immunohistochemistry and were analyzed by statistical methods to discuss their correlations.RESULTS Positive rate of Cx43,SCF^(FBXW7),p-cyclin E1(Ser73),p-cyclin E1(Thr77)and p-cyclin E1(Thr395)in detected samples were 76.32%,76.32%,65.79%,5.26%and 55.26%respectively.Positive expressions of these proteins were not related to the tissue type,degree of tissue differentiation or lymph node metastasis.Cx43 and SCF^(FBXW7)(r=0.749),p-cyclin E1(Ser73)(r=0.667)and p-cyclin E1(Thr395)(r=0.457),SCF^(FBXW7) and p-cyclin E1(Ser73)(r=0.703)and p-cyclin E1(Thr395)(0.415)were correlated in colon cancer(P<0.05),and expressions of the above proteins were positively correlated in colon cancer.CONCLUSION Cx43 may facilitate the phosphorylation of cyclin E1 at the Ser73 and Thr195 sites through its interaction with SCF^(FBXW7),thereby influencing the ubiquitination and degradation of cyclin E1.

关 键 词：Colon cancer CX43 SCF^(FBXW7) Phosphorylation of cyclin E1 Sites of cyclin E1 Correlation analysis 

分 类 号：R735.35[医药卫生—肿瘤]