Immunohistochemical expression of matrix metalloproteinase-9 and 13 in oral squamous cell carcinoma and their role in predicting lymph node metastasis  

作　　者：Bhari Sharanesha Manjunatha Keshav T Handge Vandana Sandeep Shah Yasser Eid Al-Thobaiti Deepak Gowda Sadashivappa Pateel 

机构地区：[1]Department of Basic Oral Medicine and Allied Dental Sciences,Taif University,At`Taif 26571,Makkah,Saudi Arabia [2]Department of Oral and Maxillofacial Pathology,Dr.Vasantrao Pawar Medical College,Hospital and Research Centre,Nashik 423101,Maharashtra,India [3]Department of Oral Pathology and Microbiology,KM Shah Dental College and Hospital,Sumandeep Vidyapeeth,Vadodara 391760,Gujarat,India [4]Department of Oral and Maxillofacial Surgery and Diagnostic Sciences,Faculty of Dentistry,Taif University,Al-Haweiah 26571 Makkah,Saudi Arabia [5]Department of Oral Pathology and Microbiology,Faculty of Dentistry,MAHSA University,Selangor 42610,Malaysia

出　　处：《World Journal of Methodology》2025年第2期108-116,共9页世界方法学杂志（英文）

摘　　要：BACKGROUND One of the main characteristics of oral squamous cell carcinoma(OSCC)is that it metastasizes to cervical lymph nodes frequently with a high degree of local invasiveness.A primary feature of malignant tumors is their penetration of neighboring tissues,such as lymphatic and blood arteries,due to the tumor cells'capacity to break down the extracellular matrix(ECM).Matrix metalloproteinases(MMPs)constitute a family of proteolytic enzymes that facilitate tissue remodeling and the degradation of the ECM.MMP-9 and MMP-13 belong to the group of extracellular matrix degrading enzymes and their expression has been studied in OSCC because of their specific functions.MMP-13,a collagenase family member,is thought to play an essential role in the MMP activation cascade by breaking down the fibrillar collagens,whereas MMP-9 is thought to accelerate the growth of tumors.Elevated MMP-13 expression has been associated with tumor behavior and patient prognosis in a number of malignant cases.AIM To assess the immunohistochemical expression of MMP-9 and MMP-13 in OSCC.METHODS A total of 40 cases with histologically confirmed OSCC by incisional biopsy were included in this cross-sectional retrospective study.The protocols for both MMP-9 and MMP-13 immunohistochemical staining were performed according to the manufacturer’s recommendations along with the normal gingival epithelium as a positive control.All the observations were recorded and Pearson’sχ²test with Fisher exact test was used for statistical analysis.RESULTS Our study showed no significant correlation between MMP-9 and MMP-13 staining intensity and tumor size.The majority of the patients were in advanced TNM stages(III and IV),and showed intense expression of MMP-9 and MMP-13.CONCLUSION The present study suggests that both MMP-9 and MMP-13 play an important and independent role in OSCC progression and invasiveness.Intense expression of MMP-9 and MMP-13,irrespective of histological grade of OSCC,correlates well with TNM stage.Consequently,it is evident that M

关 键 词：Matrix metalloproteinases Oral squamous cell carcinoma Tumor staging IMMUNOHISTOCHEMISTRY INVASION Lymph node metastasis TNM stage 

分 类 号：R736.1[医药卫生—肿瘤]