Preliminary study on the preparation of lyophilized acellular nerve scaffold complexes from rabbit sciatic nerves with human umbilical cord mesenchymal stem cells  

作　　者：Chuang Qian Shang-Yu Guo Zheng Xu Zhi-Qiang Zhang Hao-Dong Li Hao Li Xiong-Sheng Chen 

机构地区：[1]Department of Orthopedics,Children’s Hospital of Fudan University&National Children’s Medical Center,Shanghai 201102,China [2]Spine Center,Department of Orthopaedics,Shanghai Changzheng Hospital,Second Affiliated Hospital of Naval Medical University,Shanghai 200003,China [3]Department of Neurosurgery,Children’s Hospital of Fudan University&National Children’s Medical Center,Shanghai 201102,China

出　　处：《World Journal of Stem Cells》2024年第12期1047-1061,共15页世界干细胞杂志（英文）

摘　　要：BACKGROUND The gold standard of care for patients with severe peripheral nerve injury is autologous nerve grafting;however,autologous nerve grafts are usually limited for patients because of the limited number of autologous nerve sources and the loss of neurosensory sensation in the donor area,whereas allogeneic or xenografts are even more limited by immune rejection.Tissue-engineered peripheral nerve scaffolds,with the morphology and structure of natural nerves and complex biological signals,hold the most promise as ideal peripheral nerve“replacements”.AIM To prepare allogenic peripheral nerve scaffolds using a low-toxicity decellularization method,and use human umbilical cord mesenchymal stem cells(hUCMSCs)as seed cells to cultivate scaffold-cell complexes for the repair of injured peripheral nerves.METHODS After obtaining sciatic nerves from New Zealand rabbits,an optimal acellular scaffold preparation scheme was established by mechanical separation,varying lyophilization cycles,and trypsin and DNase digestion at different times.The scaffolds were evaluated by hematoxylin and eosin(HE)and luxol fast blue(LFB)staining.The maximum load,durability,and elastic modulus of the acellular scaffolds were assessed using a universal material testing machine.The acellular scaffolds were implanted into the dorsal erector spinae muscle of SD rats and the scaffold degradation and systemic inflammatory reactions were observed at 3 days,1 week,3 weeks,and 6 weeks following surgery to determine the histocompatibility between xenografts.The effect of acellular scaffold extracts on fibroblast proliferation was assessed using an MTT assay to measure the cytotoxicity of the scaffold residual reagents.In addition,the umbilical cord from cesarean section fetuses was collected,and the Wharton’s jelly(WJ)was separated into culture cells and confirm the osteogenic and adipogenic differentiation of mesenchymal stem cells(MSCs)and hUC-MSCs.The cultured cells were induced to differentiate into Schwann cells by the antioxidant-growth

关 键 词：Human umbilical cord mesenchymal stem cells Peripheral nerve injury Schwann cells Acellular nerve scaffolds 

分 类 号：R745[医药卫生—神经病学与精神病学]