miR-150-5p和TRAF6在重症肌无力患儿中的表达水平及意义  

Expression and significance of miR-150-5p and TRAF6 in children with myasthenia gravis

作　　者：崔艳杰 张兰兰 CUI Yanjie;ZHANG Lanlan(Department of Critical Medicine,Handan Maternal And Child Health Center,Handan Hebei 056001,China)

机构地区：[1]邯郸市妇幼保健院儿童院区重症医学科,河北邯郸056001

出　　处：《中国急救复苏与灾害医学杂志》2024年第12期1631-1634,共4页China Journal of Emergency Resuscitation and Disaster Medicine

基　　金：河北省医学科学研究计划(编号:20191808)。

摘　　要：目的探讨微小RNA-50-5p(miR-150-5p)和肿瘤坏死因子受体相关因子6(TRAF6)在重症肌无力(MG)患儿血清中的表达水平及意义。方法选取2021年6月至2022年10月本院收取的60例MG患儿(观察组)和60例健康儿童(对照组)。用定量重症肌无力评分(QMG)评估MG患儿的病情严重程度,用改良的Osserman分型法对MG进行分型。RT-PCR检测血清miR-150-5p表达水平,ELISA法检测血清TRAF6、乙酰胆碱受体(AchR)抗体、白细胞介素4(IL-4)、γ-干扰素(IFN-γ)水平,流式细胞术检测浆细胞样树突状细胞(pDC)、调节性T细胞(Treg)水平。相关性分析用Spearman秩相关分析。用受试者工作特征(ROC)曲线分析血清miR-150-5p和TRAF6诊断MG的价值,计算曲线下面积(AUC)、灵敏度和特异度。结果与对照组比较,观察组miR-150-5p、TRAF6、AchR抗体、IFN-γ水平较高,而pDC、Treg、IL-4较低(t=7.883、10.485、44.745、5.344、22.346、8.428、6.116,P<0.05)。在MG患儿血清中,miR-150-5p水平分别与TRAF6、AchR抗体、IFN-γ水平呈正相关(r=0.576、0.456、0.470,P<0.05),与pDC、Treg、IL-4水平呈负相关(r=-0.607、-0.454、-0.584,P<0.05);TRAF6水平分别与AchR抗体、IFN-γ水平呈正相关(t=0.505、0.445,P<0.05),与pDC、Treg、IL-4水平呈负相关(r=-0.432、-0.612、-0.512,P<0.05)。MG分型越高,miR-150-5p和TRAF6水平越高(F=3.694、6.487,P<0.05)。血清miR-150-5p、TRAF6水平分别与QMG评分呈正相关(r=0.664、0.589,P<0.05)。miR-150-5p联合TRAF6诊断MG的AUC为0.924,灵敏度为93.23%,特异度为83.23%。结论MG患儿血清miR-150-5p和TRAF6表达水平升高并且均与免疫损伤相关,二者有助于早期诊断MG。Objective To investigate the expression level and significance of microRNA-50-5p(miR-150-5p)and tumor necrosis factor receptor related factor 6(TRAF6)in serum of children with myasthenia gravis(MG).Methods From June 2021 to October 2022,60 MG children(observation group)and 60 healthy children(control group)were selected.The severity of MG was evaluated by quantitative myasthenia gravis score(QMG),and MG was classified by modified Osserman classification.The expression level of miR-150-5p in serum was detected by RT-PCR,and serum TRAF6,acetylcholine receptor(AchR)antibody,interleukin-4(IL-4)γ-Interferon(IFN-γ)The levels of plasma cell like dendritic cells(pDC)and regulatory T cells(Treg)were detected by flow cytometry.Spearman rank correlation analysis for correlation analysis.The diagnostic value of serum miR-150-5p and TRAF6 in MG was analyzed with the subject operating characteristic curve(ROC),and the area under the curve(AUC),sensitivity and specificity were calculated.Results Compared with the control group,in the observed group,miR-150-5p,TRAF6,AchR antibody,and IFNγlevels were higher,while pDC,Treg,and IL-4 were lower(t=7.883,10.458,44.745,5.344,22.346,8.428,6.116,P<0.05).In the serum of MG children,the level of miR-150-5p was positively correlated with the level of TRAF6,AchR antibody and IFN-γlevels,respectively(r=0.576,0.456,0.470,P<0.05),but negatively associated with pDC,Treg,and IL-4 levels,respectively(r=-0.610,-0.454,-0.584,P<0.05).The TRAF6 levels were positively correlated with AchR antibody and IFN-γlevels,respectively(r=0.576,0.456,0.470,P<0.05),but negatively associated with pDC,Treg,and IL-4 levels,respectively(r=-0.432,-0.612,-0.512,P<0.05).The higher the MG subtyping,the higher the miR-150-5p and TRAF6 levels(r=0.664,0.589,P<0.05).Serum miR-150-5p and TRAF6 levels were positively correlated with the QMG scores,respectively(F=3.694,6.487,P<0.05).The combination of miR 150-5p with TRAF6 diagnosed MG with an AUC of 0.924,a sensitivity of 93.23%,and a specificity of 83.23%.Conclusion Serum

关 键 词：微小RNA 肿瘤坏死因子受体相关因子6 重症肌无力 免疫 诊断 

分 类 号：R746.1[医药卫生—神经病学与精神病学]