Heat stress-induced decapping of WUSCHEL mRNA enhances stem cell thermotolerance in Arabidopsis  

作　　者：Sumei Liu Haijun Wu Zhong Zhao 

机构地区：[1]MOE Key Laboratory for Cellular Dynamics,Hefei National Research Center for Physical Sciences at the Microscale,The First Affiliated Hospital of University of Science and Technology of China,School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,China [2]Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations,Gansu Province Key Laboratory of Gene Editing for Breeding,School of Life Sciences,Lanzhou University,Lanzhou 730000,China

出　　处：《Molecular Plant》2024年第12期1820-1832,共13页分子植物（英文版）

基　　金：National Natural Science Foundation of China(grant nos.32321001 and 32130009 to Z.Z.);University of Science and Technology of China Research Funds of the Double First-Class Initiative(grant no.YD9100002025 to Z.Z.).

摘　　要：The plasticity of stem cells in response to environmental change is critical for multicellular organisms.Here,we show that MYB3R-like directly activates the key plant stem-cell regulator WUSCHEL(WUS)by recruiting the methyltransferase ROOT INITIATION DEFECTIVE 2(RID2),which functions in m7G methylation of the 5′cap of WUS mRNA to protect it from degradation.Transcriptomic and molecular analyses showed that protein-folding genes are repressed by WUS to maintain precise protein synthesis in stem cells by preventing the reuse of misfolded proteins.Interestingly,we found that upon heat stress,the MYB3R-like/RID2 module is repressed to reduce WUS transcript abundance through decapping of nascent WUS mRNA.This releases the inhibition of protein-folding capacity in stem cells and protects them from heat shock by eliminating misfolded protein aggregation.Taken together,our results reveal a strategic trade-off whereby plants reduce the accuracy of protein synthesis in exchange for the survival of stem cells at high temperatures.

关 键 词：WUSCHEL DECAPPING MYB3R-like METHYLTRANSFERASE THERMOTOLERANCE 

分 类 号：Q94[生物学—植物学]