Probing and imaging phospholipid dynamics in live cells  

作　　者：Zhongsheng Wu Yongtao Du Tom Kirchhausen Kangmin He 

机构地区：[1]State Key Laboratory of Molecular Developmental Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Department of Cell Biology,Harvard Medical School,Boston,MA 02115,United States [4]Program in Cellular and Molecular Medicine,Boston Children’s Hospital,Boston,MA 02115,United States [5]Department of Pediatrics,Harvard Medical School,Boston,MA 02115,United States

出　　处：《Life Metabolism》2024年第4期13-27,共15页生命代谢（英文）

基　　金：supported by the National Natural Science Foundation of China(92354305,32321004,and 91957106 to K.H.);the Ministry of Science and Technology of the People’s Republic of China(2022YFA1304500 to K.H.).

摘　　要：Distinct phospholipid species display specific distribution patterns across cellular membranes,which are important for their struc-tural and signaling roles and for preserving the integrity and functionality of the plasma membrane and organelles.Recent advance-ments in lipid biosensor technology and imaging modalities now allow for direct observation of phospholipid distribution,trafficking,and dynamics in living cells.These innovations have markedly advanced our understanding of phospholipid function and regulation at both cellular and subcellular levels.Herein,we summarize the latest developments in phospholipid biosensor design and applica-tion,emphasizing the contribution of cutting-edge imaging techniques to elucidating phospholipid dynamics and distribution with unparalleled spatiotemporal precision.

关 键 词：PHOSPHOLIPID BIOSENSOR lipid-binding domain live-cell imaging 

分 类 号：Q25[生物学—细胞生物学]