不同中医证型结直肠癌患者外周血miR-21、IL-12、T淋巴细胞亚群水平变化及临床意义  

Changes and clinical significance of miR-21,IL-12and T lymphocyte subsets in peripheral blood of patients with colorectal cancer with different TCM syndrome types

作　　者：王增辉 孙泽辉 李昊楠 郑广茂 WANG Zenghui;SUN Zehui;LI Haonan(Department of General Surgery,Tangshan Vocational and Technical College Affiliated Hospital,Tangshan Hebei 063000,China)

机构地区：[1]唐山职业技术学院附属医院普外一科,河北唐山063000 [2]张家口市第一医院消化科二科,河北张家口075000

出　　处：《四川中医》2024年第12期17-19,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：张家口市重点研发计划项目(编号:22221052D)。

摘　　要：目的:分析结直肠癌患者中医辨证分型与外周血微小RNA(miR)-21、白细胞介素(IL)-12、T淋巴细胞亚群水平变化及临床意义。方法:回顾性分析2020年2月~2023年8月我院肿瘤科收治的204例结直肠癌患者的病历资料,根据患者中医证型分为湿热内蕴证58例、脾虚气滞证41例、脾肾两虚证39例、气血两虚证37例、瘀毒内阻证29例。比较不同中医证型结直肠癌患者外周血miR-21、IL-12、T淋巴细胞亚群水平。采用Pearson相关性分析结直肠癌中医证型与外周血miR-21、IL-12、CD3^(+)、CD3^(+)CD4^(+)T的相关性。结果:5种证型结直肠癌患者血清miR-21、IL-12、CD3^(+)、CD3^(+)CD4^(+)T比较,差异有统计学意义(P<0.05)。脾肾两虚证患者血清miR-21、CD3^(+)、CD3^(+)CD4^(+)T水平均低于瘀毒内阻证、气血两虚证、脾虚气滞证、湿热内蕴证患者(P<0.05),IL-12水平均高于其他4种证型患者(P<0.05)。Pearson相关性分析结果显示,结直肠癌中医证型与miR-21、CD3^(+)、CD3^(+)CD4^(+)T均呈正相关,与IL-12呈负相关(P<0.05)。结论:结直肠癌患者中湿热内蕴证占比最高,其次为脾虚气滞证。不同中医证型患者外周血miR-21、IL-12、CD3^(+)、CD3^(+)CD4^(+)T水平存在差异,可为结直肠癌的中医辨证分型及病情评估提供参考。Objective:To study TCM syndrome differentiation and peripheral blood microrna(miR-21),interleukin(IL)-12and T lymphocyte subsets in patients with colorectal cancer and its clinical significance.Method:The medical records of 204patients with colorectal cancer admitted to Oncology Department of our hospital from February 2020to August 2023were retrospectively analyzed.According to TCM syndrome types,the patients were divided into 58cases of dampness-heat syndrome,41cases of spleen deficiency qi stagnation syndrome,39cases of spleen and kidney deficiency syndrome,37cases of qi and blood deficiency syndrome,and 29cases of internal blockage of blood stasis.To compare the levels of miR-21,IL-12 and T lymphocyte subsets in peripheral blood of colorectal cancer patients with different TCM syndrome types.Pearson correlation was used to analyze the correlation between TCM syndrome types of colorectal cancer and peripheral blood miR-21,IL-12,CD3^(+),CD3^(+)CD4^(+)T.Results:There were significant differences in serum miR-21,IL-12,CD3^(+),CD3^(+)CD4^(+)T among 5types of colorectal cancer patients(P<0.05).The serum levels of miR-21,CD3^(+),CD3^(+)CD4^(+)T in patients with spleen-kidney deficiency syndrome were lower than those in patients with internal blockage of stasis,deficiency of qi and blood,spleen deficiency qi stagnation syndrome,and internal dampness-heat syndrome(P<0.05),and the levels of IL-12 were higher than those in patients with other 4types of syndrome(P<0.05).The results of Pearson correlation analysis showed that the TCM syndrome type of colorectal cancer was positively correlated with miR-21,CD3^(+),CD3^(+)CD4^(+)T,and negatively correlated with IL-12(P<0.05).Conclusion:Damp-heat syndrome is the highest in colorectal cancer patients,followed by spleen deficiency qi stagnation syndrome.The levels of miR-21,IL-12,CD3^(+),CD3^(+)CD4^(+)T in peripheral blood of patients with different TCM syndrome types are different,which can provide reference for TCM syndrome differentiation and disease evaluation of colorecta

关 键 词：结直肠癌 中医证型 微小RNA 白细胞介素 淋巴细胞亚群 

分 类 号：R735.37[医药卫生—肿瘤]