Lactose hydrolyzing activity of the lactase immobilized polycaprolactone and silk fibroin-based nanofiber and nitrocellulose membrane  

作　　者：Sümeyye Yılmaz-Karaoglu Begüm Gürel-Gokmen Tugba Tunali-Akbay 

机构地区：[1]Vocational School of Health Services,Fenerbahce University,Istanbul,Turkey [2]Department of Basic Medical Sciences,Dentistry Faculty,Marmara University,Istanbul,Turkey

出　　处：《Food Bioscience》2022年第5期569-575,共7页食品生物科学（英文）

基　　金：Marmara University Scientific Research Project(TYL-2021-10180)units.

摘　　要：This study aimed to investigate the activity of lactase immobilized polycaprolactone and silk fibroin(PCL/SF)-based nanofiber and nitrocellulose membrane for the preparation of lactose-reduced milk.PCL/SF-based nanofiber was prepared by using the electrospinning method.The lactase enzyme was immobilized using the physical adsorption method on both surfaces,and then the optimum operating temperature and pH of the immobilized enzymes were determined.The efficiency of the immobilized lactase enzyme was determined in both goat and cow milk.The nutrient content of milk was also analyzed before and after the incubation of nanofiber and membrane with milk.The lactose hydrolysis efficiency of the lactase immobilized nitrocellulose membrane was found to be higher than the lactase immobilized PCL/SF-based nanofiber.Lactose was hydrolyzed 59%in cow milk and 87%in goat milk by using lactase immobilized nitrocellulose membrane.42%of lactose was also hydrolyzed in cow milk and 21%was hydrolyzed in goat milk by using lactase immobilized PCL/SF-based nanofiber.However,the use of these two bioactive surfaces did not change the fat and protein composition of both cow and goat milk.In conclusion,lactase immobilized nitrocellulose membrane was found to be more advantageous in the production of lactose-reduced milk than the lactase immobilized polycaprolactone/silk fibroin nanofiber.

关 键 词：Lactose reduced milk IMMOBILIZATION Bioactive surface Electrospinning Lactose intolerance 

分 类 号：TS201.2[轻工技术与工程—食品科学]