Evaluating the phyto-complexity and poly-pharmacology of spices: The case of Aframomum melegueta K. Schum (Zingiberaceae)  

作　　者：Simon Vlad Luca Adriana Trifan Gokhan Zengin Kouadio Ibrahim Sinan Abdullahi Ibrahim Uba Izabela Korona-Glowniak Krystyna Skalicka-Wozniak 

机构地区：[1]Biothermodynamics,TUM School of Life Sciences,Technical University of Munich,85354,Freising,Germany [2]Department of Pharmacognosy,Grigore T.Popa University of Medicine and Pharmacy Iasi,700115,Iasi,Romania [3]Physiology and Biochemistry Research Laboratory,Department of Biology,Science Faculty,Selcuk University,University Campus,42130,Konya,Turkey [4]Department of Molecular Biology and Genetics,Faculty of Engineering and Natural Sciences,Kadir Has University,Istanbul,34083,Turkey [5]Department of Pharmaceutical Microbiology,Faculty of Pharmacy,Medical University of Lublin,20-093,Lublin,Poland [6]Department of Natural Products Chemistry,Medical University of Lublin,20-093,Lublin,Poland

出　　处：《Food Bioscience》2022年第5期1230-1245,共16页食品生物科学（英文）

摘　　要：The seeds of Aframomum melegueta K. Schum (grains of paradise, Zingiberaceae) are used as a common spice in African countries and a fine condiment in the European cuisine. In this study, we evaluated the phytochemical profile of various seed extracts of A. melegueta as well as their antimicrobial, antioxidant and enzyme inhibitory effects. In total, 25 diarylheptanoids, five gingerol derivatives and nine phenolic/organic acids were tentatively annotated in A. melegueta by liquid chromatography hyphenated with high resolution tandem mass spectrometry (LC-HRMS/MS). A. melegueta showed a moderate inhibitory activity against different human pathogenic microbial strains, with H. pylori the most sensitive microorganism. A strong antioxidant and enzyme inhibitory potential was shown in various radical scavenging, reducing and chelating assays as well as in cholinesterase, tyrosinase and glucosidase assays. Several specialized metabolites from A. melegueta (diarylheptanoids, gingerols) were shown to be directly linked with the investigated antioxidant and enzyme inhibitory activities, as evaluated through the correlation analysis. In addition, two diarylheptanoids (one heptan-3-ol and one heptan- 3,5-diol) displayed the strongest binding to acetylcholinesterase (AChE) via multiple H-bonds, a couple of π-π interactions and van der Waals interactions all over the catalytic channel of the enzyme, as evidenced by the molecular docking study. Overall, our work brings new contributions to the phyto-complexity and poly-pharmacology of spices from genus Aframomum than can find future applications in pharmaceutical, nutraceutical or cosmeceutical industry.

关 键 词：Aframomum melegueta ZINGIBERACEAE SPICES Gingerols HELICOBACTER 

分 类 号：O62[理学—有机化学]