Piperine attenuates production of inflammatory biomarkers,oxidative stress and neutrophils in lungs of cigarette smoke-exposed experimental mice  

作　　者：Poonam Arora Seyyed Shamsadin Athari Lalit Mohan Nainwal 

机构地区：[1]Department of Pharmacognosy and Phytochemistry,School of Pharmaceutical Education and Research,Jamia Hamdard,New Delhi,India [2]Department of Pharmacognosy and Phytochemistry,SGT College of Pharmacy,SGT University,Gurugram,Haryana,India [3]Department of Immunology,School of Medicine,Zanjan University of Medical Sciences,Zanjan,Iran [4]Department of Pharmaceutical Chemistry,School of Pharmaceutical Education and Research,Jamia Hamdard,New Delhi,India [5]Department of Pharmacy,School of Medical&Allied Sciences,G.D.Goenka University,Gurugram,Haryana,India

出　　处：《Food Bioscience》2022年第5期1285-1298,共14页食品生物科学（英文）

基　　金：Notice for SRF,45/48/2011/BMS/TRM,dated 28-05-2013.Indian Council of Medical Research(ICMR),India.

摘　　要：Cigarette smoking(CS)induced chronic obstructive pulmonary disease(COPD)is third leading cause of global pulmonary health issue.Currently,there is no effective therapeutic strategy to control perpetuating inflammatory response in COPD.The aim of current study was to investigate the protective effects of piperine in CS-induced murine model of COPD.Exposure of experimental animals to cigarettes smoke twice daily for 10 weeks resulted in increased production of pro-inflammatory mediators including IL-1β,IL-8,TNF-α,LTB-4 and neutrophil elastase(NE)in bronchoalveolar(BAL)fluid.Oxidative stress biomarkers,malondialdehyde(MDA),myeloperoxidase(MPO),nitric oxide and MMP9 also significantly increased in CS-control mice lungs as compared to normal control group(P<0.001).Moreover,CS also increased recruitment of inflammatory cells,neutrophils and macrophages into BAL fluid and altered breathing rate and tidal volume as compared to normal control mice.However,oral treatment of CS-exposed animals with test drug,piperine(10 mg/kg,b.w.or 20 mg/kg,b.w.)or reference standard methyl prednisolone(3.25 mg/kg,b.w.)for 21 days or 7 days,respectively,significantly(p<0.05,0.01,0.001)improved pulmonary function and reversed CS-induced production of cytokines,oxidative stress biomarkers and other biochemicals as compared to CS-exposed control mice.Further,In silico molecular docking studies exhibited attractive binding affinity of piperine for human neutrophil elastase,MMP-9,MPO and IL-8 receptors.Findings of our study suggest protective effect of piperine in experimental animals by repressing CS-induced infiltration of inflammatory cells and thereby exaggerated production of pro-inflammatory mediators and oxidative stress.

关 键 词：PIPERINE Cigarettes smoke Chronic obstructive pulmonary disease(COPD) Oxidative stress Pro-inflammatory mediators 

分 类 号：R96[医药卫生—药理学]