Optimization for the extraction of polysaccharides from Huidouba and their in vitroα-glucosidase inhibition mechanism  

作　　者：Yuxin Wen Xin Zhou Da Huo Juncheng Chen Longmei Weng Bing Li Zhiqiang Wu Xia Zhang Lin Li 

机构地区：[1]School of Food Science and Engineering,Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety,Engineering Research Center of Starch and Plant Protein Deep Processing,Ministry of Education,South China University of Technology,Guangzhou,510640,China [2]School of Chemical Engineering and Energy Technology,Dongguan University of Technology,Dongguan,523808,China

出　　处：《Food Bioscience》2022年第5期1334-1341,共8页食品生物科学（英文）

基　　金：supported by the National Key R&D Program of China(no.2016YFD0400303);Guangdong Province YangFan Innovative and Entrepreneurial Research Team Project(no.2014YT02S029).

摘　　要：Huidouba is a kind of fungus plant that grows by spiders parasitizing tea trees at an altitude of 1500 m above Mount Emei.The bioactive polysaccharides extracted from Huidouba have been traditionally used in the treatment of diabetes in China.In this study,the optimal hot-water extraction condition of crude Huidouba polysaccharides was obtained using Box-Behnken design:the temperature of 93.7◦C,the time of 3.57 h,the liquid-to-solid ratio of 28.47 mL/g,and the yield was 0.46%.Three purified Huidouba polysaccharides(HDBPs,including HDBP-d,HDBP-1,and HDBP-2)were separated through deproteinization,ultrafiltration,and column chromatography.Then the inhibition mechanism and kinetics of HDBPs againstα-glucosidase were investigated.The inhibitory activity of HDBPs onα-glucosidase was positively correlated with their concentration,and their mechanism of quenching fluorescence was ascertained to be the static type induced by the formation of HDBPs-α-glucosidase complex.HDBP-d and HDBP-2 inhibited the activity ofα-glucosidase in a reversible competitive-type manner while HDBP-1 was a noncompetitive-type inhibitor.The inhibitory activities of HDBPs onα-glucosidase correlated inversely with temperature in most cases,and showed weaker inhibitory activity at extreme acidic or alkaline conditions.These findings highlight the efficacy,kinetics and mechanism of HDBPs inhibitingα-glucosidase in vitro.The study provided a replaceable approach for chemical inhibitors ofα-glucosidase with natural fungus plant extracts,and suggested the theoretical basis that the plant-derived polysaccharides with glucosidase inhibition effects were applied in medicines.

关 键 词：HUIDOUBA POLYSACCHARIDES EXTRACTION Α-GLUCOSIDASE Enzyme kinetic 

分 类 号：TS201.2[轻工技术与工程—食品科学]