非小细胞肺癌患者中慢拉法与抽吸法对应用EBUS-TBNA行NGS检测效果的比较  

Comparison of the Methods of Aspiration and Slow-pull in Endobronchial Ultrasound-guided Transbronchial Needle Aspiration for Next Generation Sequencing-compatible Tissue Collection in Patients with Non-small Cell Lung Cancer

作　　者：王乔蓓 朱琳[1] 韩觉明 李彩瑜 冯菲菲 WANG Qiaobei;ZHU Lin;HAN Jueming;LI Caiyu;FENG Feifei(Department of Pulmonary and Critical Care Medicine,The Second Hospital of Shandong University,Ji'nan 250033,China)

机构地区：[1]山东大学第二医院呼吸与危重症医学科,山东济南250033

出　　处：《药物生物技术》2024年第5期519-522,共4页Pharmaceutical Biotechnology

基　　金：国家自然科学基金青年科学基金项目(No.82103803)。

摘　　要：探讨慢拉法与抽吸法取样在应用超声支气管镜引导下的经支气管镜针吸活检术(EBUS-TBNA)对非小细胞肺癌患者行二代测序(NGS)检测质量的影响。选取2018年1月—2022年12月于医院就诊的非小细胞肺癌患者82例,按照取样方法分为慢拉法组(40例)和抽吸法(42)组。评估两组间样本的组织量、血液污染程度、肿瘤细胞数量和NGS成功率。两组患者在年龄、性别、肿瘤部位、肿瘤分期、病理类型等方面均无统计学差异(P>0.05)。比较分析发现,慢拉法与抽吸法相比能够得到的肺组织样本质量更高(60.0%vs 47.6%,P=0.034)、血液污染程度更低(5.0%vs 7.0%,P=0.009)、包含的肿瘤细胞更多(中位数339个vs 106个,P<0.001),并且使用慢拉法(95.0%)得到的样本行NGS检测的成功率亦高于抽吸法(69.1%)。本研究结果提示,慢拉法取样有助于非小细胞肺癌患者行EBUS-TBNA获取更高质量的用于NGS检测的组织样本,可用于更精准地指导患者的个体化治疗。To compare the methods of aspiration and slow-pull in endobronchial ultrasound-guided transbronchial needle aspiration(EBUS-TBNA)for next generation sequencing(NGS)-compatible tissue collection in patients with non-small cell lung cancer(NSCLC),a total of 82 patients with NSCLC were enrolled from January 2020 to December 2022.The patients were divided into slow-pull group(n=40)and aspiration group(n=42)according to the collection method.The presence of tissue cores,degree of blood contamination,number of tumor cells and the success rate of NGS were compared between the two groups.There was no signifi-cant difference of age,sex,location of tumor,stage of tumor,and pathological diagnosis between the two groups(P>0.05).The slow-pull group had higher yield of tissue cores(60.7%vs 47.2%,P=0.034),lower degree of blood contamination(5.0%vs 7.1%,P=0.009),and higher number of tumor cells(median 339 counts vs 106 counts,P<0.001)than the aspiration method.The success rate of the NGS was also significantly higher in the slow-pull group(95.0%)than those in the aspiration group(69.1%)(P<0.001).Taken together,the results of the present study indicate that the slow-pull method might be a superior tech-nique for EBUS-TBNA to obtain high-quality tissue samples for NGS in patient with NSCLC.The slow-pull method may therefore contribute to the identification of driver gene mutations and translocations and facilitate personalized treatment of NSCLC.

关 键 词：非小细胞肺癌 二代测序 超声支气管镜引导下的经支气管镜针吸活检术 慢拉法 抽吸法 

分 类 号：R734.2[医药卫生—肿瘤]