枯草芽孢杆菌XE-2的分离鉴定及生物学特性分析  

Isolation,Identification and Biological Characteristics Analysis of Bacillus subtilis in Soil

作　　者：杜丽娜 檀冰凝 任桐瑶 徐一帆 李亚子 许可 张运峰[1] 范永山[1] DU Li-na;TAN Bing-ning;REN Tong-yao;XU Yi-fan;LI Ya-zi;XU Ke;ZHANG Yun-feng;FAN Yong-shan(Department of Life Sciences,Tangshan Normal University,Hebei Key Laboratory of Plant Biotechnology Research and Application,Tangshan 063000,China)

机构地区：[1]唐山师范学院生命科学系,河北省植物生物技术研究与应用重点实验室,河北唐山063000

出　　处：《唐山师范学院学报》2024年第6期47-50,73,共5页Journal of Tangshan Normal University

基　　金：中央引导地方科技发展资金项目(246Z3610G);唐山师范学院科学研究基金项目(2023B08)。

摘　　要：从唐山市丰润区某中学校园内田地根际土壤中分离出枯草芽孢杆菌XE-2,再对其进行生长曲线测定、药物敏感性试验以及抑菌试验。结果表明,枯草芽孢杆菌XE-2培养0-1h为生长延迟期,1-16h为生长对数期,16-24h为生长平稳期。XE-2对头孢吡肟、利福平和苯唑西林等14种药物均敏感,对头孢他啶和克林霉素中介,对蜡样芽孢杆菌、大肠杆菌和苏云金杆菌均有一定的抑制作用。This experiment conducted growth curve determination,drug sensitivity test,and antibac-terial test on Bacillus subtilis XE-2 isolated from the field rhizosphere soil of a middle school campus in Fengrun District,Tangshan City.The results of the growth curve indicated that B.subtilis XE-2 has a de-lay period of 0-1 h,a logarithmic period of 1-16 h,and a stationary period of 16-24 h.The drug sensitivity test results showed that XE-2 is sensitive to 14 drugs,including cefepime,rifampicin,and oxacillin,and mediates with ceftazidime and clindamycin.The antibacterial test results showed that XE-2 has inhibitory effects on Bacillus cereus,Escherichia coli,and Bacillus thuringiensis.

关 键 词：枯草芽孢杆菌 16SrDNA 药敏试验 抑菌试验 生长曲线 

分 类 号：R155[医药卫生—营养与食品卫生学]