基于iTRAQ蛋白质组学技术分析羧甲基茯苓多糖对老龄小鼠免疫功能和血脂调节的机理  

Mechanisms of Carboxymethylpachymaran on Regulation of Immune Functions and Plasma Lipid in Aged Mice By iTRAQ-Based Proteomic Technology

作　　者：徐晓飞 陈萌 谢英洁 吴丰魏 XU Xiaofei;CHEN Meng;XIE Yingjie;WU Fengwei(College of Food Science and Engineering,Guangdong Ocean University,Yangjiang 529500,China)

机构地区：[1]广东海洋大学食品科学与工程学院,广东阳江529500

出　　处：《食品科学技术学报》2024年第6期54-67,共14页Journal of Food Science and Technology

基　　金：广东海洋大学科研启动经费项目(36030202062207)。

摘　　要：大量研究证明,真菌多糖具有抗衰老的潜力,然而真菌多糖抗衰老的生物学机制尚未完全阐明。茯苓是我国传统药食两用真菌资源,多糖是其主要活性成分。以每天50 mg/kg羧甲基茯苓多糖(carboxymethylpachymaran,CMP)灌胃12月龄小鼠连续30 d,评估CMP对老龄小鼠免疫功能和血脂的影响,并利用基于相对和绝对定量的同位素标签(isobaric tags for relative and absolute quantification,iTRAQ)的蛋白质组学技术分析小鼠小肠组织蛋白表达水平的变化。结果表明:CMP提高了老龄小鼠的胸腺指数、脾淋巴细胞增殖活性和自然杀伤细胞的杀伤活性;增加了其血清I L-6和I L-10水平,降低了其血清总胆固醇(TC)、总甘油三酯(TG)和低密度脂蛋白胆固醇(LD L-C),并提高了高密度脂蛋白胆固醇(HD L-C)水平,体现出CMP部分恢复了年龄相关的免疫功能下降和血脂改变。同时,在CMP的作用下,在老龄小鼠小肠组织中共鉴定出67个差异表达蛋白,其中41个蛋白下调、26个蛋白上调。生物学信息分析表明,CMP诱导的差异表达蛋白参与的生物学功能主要是核糖体组成、蛋白质合成、免疫功能调节、脂质与能量代谢。蛋白互作分析揭示,CMP诱导的差异表达蛋白存在直接或间接的相互作用,其中热应激蛋白90β、90α,60S核糖体蛋白L4、L7、L6、L19和40S核糖体蛋白S10,异构核糖核蛋白A/B、A1,是响应CMP提高老龄小鼠免疫功能和调节血脂作用的关键蛋白。这些发现旨在为CMP应用于抗衰老食品提供参考,并加深对真菌多糖在预防衰老相关免疫功能和血脂代谢的生物学机制的理解。Numerous studies have proven that fungal polysaccharides have anti-aging potential.However,the biological mechanisms of anti-aging of fungal polysaccharides have not been fully elucidated.Poria cocos is a traditional medicinal and dietary fungal resource in China and its main active component is polysaccharides.The effects of Poria cocos-derived polysaccharides(carboxymethylpachymaran,CMP)on the immune functions and plasma lipid in 12-month-old mice were measured after oral gavage with a dosage of 50 mg/kg per day CMP for a consecutive 30 d,and then the proteome profiles in the small intestinal tissues from the aged mice were evaluated using proteomic method based on isobaric tags for relative and absolute quantification(iTRAQ)to explore the anti-aging potential of CMP and the underlying mechanisms.The results showed that CMP increased thymic index,enhanced the activities of splenic lymphocyte proliferation and natural killer(NK)cells,elevated the levels of serum I L-6 and I L-10,decreased the levels of serum total cholesterol(TC),triglyceride(TG),and low-density lipoprotein-cholesterol(LD L-C),but increased high-density lipoprotein-cholesterol(HD L-C)levels in the aged mice,indicating that CMP partially restored the age-related immune function decline and altered plasma lipid profiles.Meanwhile,under the action of CMP,a total of 67 differentially regulated proteins(DRPs)were identified in the small intestinal tissues,of which 41 proteins were down-regulated and 26 proteins were up-regulated in expression.Bioinformatic analysis elucidated that these DRPs induced by CMP were mainly involved in the biological functions of ribosome component,protein synthesis,regulation of immune function,and lipid and energy metabolism.Protein-protein interaction analysis revealed that these DRPs induced by CMP had direct and/or indirect interactions.Among them,nine DRPs,including heat shock protein HSP 90-beta and HSP 90-alpha,60S ribosomal protein L4,L7,L6 and L19,40S ribosomal protein S10,and heterogeneous nuclear ribonucleoprot

关 键 词：iTRAQ蛋白质组 茯苓 羧甲基茯苓多糖 老龄小鼠 免疫 血脂 

分 类 号：TS202.1[轻工技术与工程—食品科学]