花生籽仁抗黄曲霉菌侵染评价方法的优化及应用  

Improvement and utilization for scoring of the Aspergillus flavus infection to peanut seed

作　　者：晋高锐 喻博伦 郭建斌[1] 丁膺宾 刘念[1] 罗怀勇[1] 陈伟刚[1] 黄莉[1] 周小静[1] 雷永[1] 廖伯寿[1] 姜慧芳[1] JIN Gao-rui;YU Bo-lun;GUO Jian-bin;DING Ying-bin;LIU Nian;LUO Huai-yong;CHEN Wei-gang;HUANG Li;ZHOU Xiao-jing;LEI Yong;LIAO Bo-shou;JIANG Hui-fang(Oil Crops Research Institute,China Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,the Ministry of Agriculture and Rural Affairs,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2024年第6期1405-1411,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家现代农业产业技术体系(CARS-13);国家自然科学基金(32101708,31971903);农作物种质资源保护项目(19230873);国家科技资源共享服务平台项目(NCGRC-2023-036)。

摘　　要：花生是重要的油料作物,黄曲霉毒素污染严重影响花生食用安全和产业发展。培育和应用抗性品种是防治黄曲霉毒素污染的有效措施。然而,国内外生产上应用的抗黄曲霉花生品种极少,主要原因除了花生黄曲霉抗性鉴定过程复杂和所需设备要求高以外,评价方法不合理也影响了鉴定结果的稳定性,使得种质资源和育种材料黄曲霉抗性鉴定研究进展缓慢。本研究针对评价方法,在前期工作基础上,对花生籽仁受黄曲霉侵染等级的划分进行优化调整,将原来的4个等级(简称为“4级评价方法”)调整为9个等级(简称为“9级评价方法”)。实验结果表明,9级评价方法能更好地将不同材料的黄曲霉抗性区分开,而且鉴定结果稳定。进一步利用9级评价方法对杂交后代群体进行鉴定,并与4级评价方法的鉴定结果进行比较,发现9级评价方法发掘抗性材料的效率更高,得到的抗性QTL位点更多。研究表明,利用9级评价方法对花生籽仁进行黄曲霉抗性鉴定更为有效。Peanut is an important oil crop.However,food safety and industrial development of peanuts were limited by aflatoxin contamination.Breeding and applying resistant varieties have been effective approaches for afla⁃toxin control.However,progress in the identification of resistant germplasm resources and resistance breeding pro⁃grams was hampered by complex,costly and unreasonable methods of A.flavus infection resistance in peanut seeds.In this study,the classification of peanut seeds after infected by A.flavus in the evaluation method was optimized and adjusted based on previous methods.The previous score for A.flavus infectione to peanuts seeds with 4 levels(referred to as"4-level score")was improved to 9 levels(referred to as"9-level score").The results from evaluating peanut seeds infection with A.flavus using the 9-level score showed that it could better distinguish the difference in⁃the A.flavus infection resistance among peanut varieties compared to the 4-level score.Furthermore,the results of comparing 9-level score and 4-level score were used in screening the A.flavus infection in a hybrid progeny popula⁃tion showed,the 9-level score was more efficient in identifying resistant materials and detected more QTLs in genet⁃ic linkage analysis.These results indicate that the 9-level score is an effective and stable index for A.flavus resis⁃tance peanut evaluation.

关 键 词：花生籽仁 黄曲霉菌侵染 评价方法 优化 

分 类 号：S565.2[农业科学—作物学]