不同提取方法对当归饮片中阿魏酸含量测定的影响  

Effects of Different Extraction Methods on the Determination of Ferulic Acid Content in Angelicae Sinensis Pieces

作　　者：郑立龙[1] 杨仁婧 ZHENG Lilong;YANG Renjing(Institute of Agricultural Economics and Information,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China;Agronomy College,Gansu Agricultural University,Lanzhou Gansu 730070,China)

机构地区：[1]甘肃省农业科学院农业经济与信息研究所,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070

出　　处：《寒旱农业科学》2024年第12期1153-1156,共4页Journal of Cold-Arid Agricultural Sciences

摘　　要：通过测定不同提取方法下当归饮片中阿魏酸的含量来确定其最佳提取方法。采用浸渍法、回流提取法、超声法、水提法4种常规提取方法制备样品,选用高效液相色谱法(HPLC)测定不同提取方法制成当归提取样品中的阿魏酸含量,并以当归中阿魏酸的提取率为评价指标,对4种方法的提取效果进行比较。结果表明,使用浸渍法在室温70%乙醇溶剂中浸渍7d提取当归饮片中阿魏酸的提取率最高,为0.081%,可在实验中优先考虑。By measuring the content of ferulic acid using different extraction methods for Angelica sinensis pieces,the optimum extraction method was identified.Four conventional extraction methods were used to make samples in this experiment,including impregnation method,reflux extraction method,ultrasonic method and water extraction method,contents of ferulic acid in Angelica sinensis pieces prepared by dfferent extraction methods were determined by high performance liquid chromatography(HPLC)method.The extraction rate of ferulic acid from Angelica sinensis was used as evaluation index,the extraction effects of the four methods were compared.The results showed that the extraction rate of ferulic acid from Angelica sinensis pieces was the highest(0.081%)by immersion method in 70%ethanol solvent at room temperature for 7 days,which could be given priority in the experiment.

关 键 词：当归 阿魏酸 提取工艺 含量测定 HPLC 

分 类 号：S567.239[农业科学—中草药栽培]