基于p-AMPKα/AMPKα探讨牛磺酸组方复合黄芪提升疲劳小鼠运动能力作用机制  

Mechanism of enhancing exercise ability of compound taurine formula with Radix Astragali in mice models of fatigue based on p-AMPKα/AMPKα

作　　者：何晶 李林泽 张奇 伍小芳 赵星宇 朱映黎[1] HE Jing;LI Linze;ZHANG Qi;WU Xiaofang;ZHAO Xingyu;ZHU Yingli(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《华南预防医学》2024年第10期941-946,共6页South China Journal of Preventive Medicine

基　　金：国家重点研发计划(2018YFC1706800)。

摘　　要：目的 通过对比牛磺酸组方及牛磺酸组方复合黄芪,探讨黄芪提高牛磺酸组方对疲劳小鼠运动能力的提升及调控p-AMPKα/AMPKα的作用机制。方法 雄性昆明小鼠140只随机分为7组,每日灌胃给药1次,第14 d和第28 d天各进行1次负重游泳实验,末次给药后取小鼠组织,测量血清中乳酸(LA)、乳酸脱氢酶(LDH)、尿素氮(BUN)、肌酐(Cre)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平;检测肌肉组织中糖原含量及AMPKα和p-AMPKα表达量。结果 牛磺酸组方及牛磺酸组方复合黄芪均无肝毒性。对比同剂量牛磺酸组方,加入黄芪后小鼠力竭游泳时间增加34%;LA含量明显降低,LDH和SOD含量显著升高(P<0.05);p-AMPKα/AMPKα蛋白表达水平增加(P<0.01)。结论 牛磺酸组方复合黄芪后可减少LA和BUN积累,增加肌糖原储备,通过增加AMPKα磷酸化明显延长小鼠力竭游泳时间,提升疲劳小鼠运动能力。Objective To explore the effect of Radix Astragali on the exercise ability of fatigue mice and the regulation mechanism of p-AMPKα/AMPKα by comparing taurine formula and compound taurine formula with Radix Astragali.Methods A total of 140 male Kunming mice were randomly divided into 7 groups and given intragastrically once a day,and weight-bearing swimming experiments were carried out on the 14th and 28th days respectively.After the last administration,mice tissues were collected.Serum levels of lactic acid(LA),lactate dehydrogenase(LDH),blood urea nitrogen(BUN),creatinine(Cre),superoxide dismutase(SOD),and malondialdehyde(MDA) were measured.The content of glycogen and the expression of AMPKα and p-AMPKα in muscle tissue were detected.Results Taurine formula and compound taurine formula with Radix Astragali had no liver toxicity.Compared with taurine formula of the same dose,the exhaustive swimming time of mice increased by 34% after adding Radix Astragali;LA content was significantly decreased,LDH and SOD contents were significantly increased(P<0.05);The expression level of p-AMPKα/AMPKα protein was increased(P<0.01).Conclusion Compound taurine formula with Radix Astragali can reduce the accumulation of LA and BUN,increase muscle glycogen reserve,significantly prolong the exhaustive swimming time of mice by increasing AMPKα phosphorylation,and improve the exercise ability of fatigue mice.

关 键 词：牛磺酸 黄芪 疲劳 肌糖原 p-AMPKα/AMPKα 

分 类 号：R459.3[医药卫生—治疗学] R285.5[医药卫生—临床医学]