钛表面绿原酸涂层在高糖环境下的成骨潜能  

A self-assembled layer on titanium surface via silane coupling of chlorogenic acid improves the osteogenic potential in a high-glucose environment

作　　者：王明娟 陈晨[1,2,3] 谢海峰 WANG Mingjuan;CHEN Chen;XIE Haifeng(Deparment of Endodontics,The Afiliated Stomatological Hospial of Nanjing Medical Uniersity,Nanjing 210029,China;State Key Laboratory Cultivation Base of Research,Prevention and Treatment for Oral Disease,Nanjing 210029,China;Jiangsu Province Engineering Research Center of Stomatological Translational Medicine,Nanjing 210029,China;Department of Prosthodontics,The Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学附属口腔医院牙体牙髓科,江苏南京210029 [2]口腔疾病研究与防治国家级重点实验室培育建设点,江苏南京210029 [3]江苏省口腔转化医学工程研究中心,江苏南京210029 [4]南京医科大学附属口腔医院修复科,江苏南京210029

出　　处：《口腔生物医学》2024年第6期322-327,共6页Oral Biomedicine

基　　金：国家自然科学基金(81790927);江苏省社会发展项目(BE 2022797);江苏省科教能力提升工程——江苏省研究型医院(YJX-YYJSDW4);江苏省医学创新中心(CXZX202227)。

摘　　要：目的:探究钛表面绿原酸(CGA)涂层在高糖环境下的成骨潜能及抗氧化能力。方法:利用[3-(2-氨基乙基氨基)-丙基]三甲氧基硅烷(AEAPTMS)将CGA接枝于钛片表面从而形成生物活性涂层。采用扫描电镜、水接触角仪器对涂层进行表征分析。在高糖环境下用小鼠前成骨细胞系MC3T3-E1评价涂层对细胞增殖、黏附,成骨潜能、活性氧(ROS)生成能力及成骨相关基因表达的影响。结果:CGA成功接枝于钛表面,并具有良好的亲水性能(P<0.001);该涂层具有促进细胞的增殖及黏附能力,降低ROS生成且能促进成骨相关基因表达(P<0.05)。结论:钛表面CGA涂层在高糖环境下具有减低ROS生成能力并促进钛表面成骨的作用。Objective:To explore the osteogenic potential and antioxidant ability of chlorogenic acid(CGA)coating on titanium surface in a high glucose environment.Methods:A bioactive coating was formed on the titanium surface by grafting CGA using[3-(2-amino ethyl amino)-propyl]three oxygen radicals silane(AEAPTMS).The coating was characterized using scanning electron microscopy and a water contact angle instrument.Cell proliferation,adhesion to the coating,osteogenesis potential,reactive oxygen species(ROS)generation capability and the expression of osteogenesis-related genes was detected by real-time fluorescence quantitative PCR were evaluated in MC3T3-E1 cell line under high glucose conditions prior to ossification.Results:The successful grafting of chlorogenic acid onto the titanium surface resulted in a highly hydrophilic coating(P<0.001).This coating effectively enhances cell proliferation and adhesion,while reducing ROS generation and promoting the expression of osteogenesis-related genes(P<0.05).Conclusions:The chlorogenic acid coating on the titanium surface exhibits a potential to mitigate ROS generation and enhance osteogenesis in a high glucose environment.

关 键 词：钛植体 绿原酸 糖尿病 成骨潜能 

分 类 号：R783.4[医药卫生—口腔医学]