人腮腺区多形性腺瘤中差异表达长链非编码RNA的初步筛选分析  

Preliminary screen and analysis of differentially expressed lncRNA in human parotid pleomorphic adenoma

作　　者：许飞虎 张祥 汪旭 丁修明 徐万林 XU Feihu;ZHANG Xiang;WANG Xu;DING Xiuming;XU Wanlin(Department of Stomatology,Hai'an People's Hospital,Nantong 226600,China;Department of Otolaryngology,Hai'an People's Hospital,Nantong 226600,China;Department of Oromaxillofacial Head and Neck Oncology,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011,China)

机构地区：[1]海安市人民医院口腔科,江苏南通226600 [2]海安市人民医院耳鼻咽喉科,江苏南通226600 [3]上海交通大学医学院附属第九人民医院口腔颌面-头颈肿瘤科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海200011

出　　处：《口腔生物医学》2024年第6期341-345,共5页Oral Biomedicine

基　　金：南通市科技局社会民生科技计划项目(MSZ2022107)。

摘　　要：目的:检测人腮腺区多形性腺瘤中差异表达的长链非编码RNA(lncRNA),并进行初步筛选分析。方法:收集3对人腮腺区多形性腺瘤肿瘤(肿瘤组)和瘤旁标本(对照组),运用高通量lncRNA芯片检测其差异表达的lncRNA,统计分析差异表达lncRNA的数目和分类。通过计算Pearson相关系数,筛选出与PLAG1和CTNNB1共表达的lncRNA,并利用实时荧光定量PCR对其中感兴趣lncRNA的表达进行验证。结果:相较于对照组,共计17382个lncRNA在人腮腺区多形性腺瘤肿瘤标本中呈现显著差异(上调的8182个,下调的9200个),而差异表达lncRNA的主要为自然反义型和基因间型lncRNA(P<0.05)。同时筛选出23个与PLAG1和CTNNB1共表达的lncRNA(上调的11个,下调的12个),其中lncRNA ENST00000609511在扩大样本验证中差异无统计学意义(P>0.05),而lncRNA T256768在肿瘤标本中的表达较对照组中明显降低(P<0.05)。结论:人腮腺区多形性腺瘤中差异表达的lncRNA T256768可与腮腺区多形性腺瘤的发生发展相关。Objective:To preliminarily screen and analyze the differentially expressed long non-coding RNA in human parotid pleomorphic adenoma.Methods:Three pairs of human parotid pleomorphic adenoma tumors(tumor group)and adjacent specimens(control group)were collected and differentially expressed lncRNA were detected by high-throughput lncRNA-chip.By calculating the Pearson correlation coefficient(pcc),lncRNA co-expressed with pleomorphic adenoma gene 1(PLAG1)and catenin beta 1(CTNNB1)were screened,and the expression of interested lncRNA were verified using real-time PCR.Results:Compared to the control group,a total of 17382 lncRNA showed significant differencesin human parotid pleomorphic adenoma tumor samples;among these lncRNA,8182 were up-regulated,and 9200 were down-regulated,which were mainly anti-sense and intergenic lncRNA(P<0.05).Meanwhile,23 lncRNA co-expressed with PLAG1 and CTNNB1 were screened(11 upregulated and 12 downregulated),among which lncRNA ENST00000609511 showed no statistical difference in following validation(P>0.05),while the expression of T256768 in tumor samples was significantly reduced compared to the control group(P<0.05).Conclusions:The differential expression of lncRNA-T256768 may be related to the occurrence and development of parotid pleomorphic adenoma.

关 键 词：多形性腺瘤 腮腺 唾液腺肿瘤 长链非编码RNA 多形性腺瘤基因1 

分 类 号：R739.8[医药卫生—肿瘤]