基于合成生物学微生物底盘细胞构建与优化的研究进展  

Advances in Microbial Genome Reduction and Optimization Based on Synthetic Biology

作　　者：孙天丽 黄明珠 刘斌 陈雪岚[1,2] SUN Tianli;HUANG Mingzhu;LIU Bin;CHEN Xuelan(College of Life Science,Jiangxi Normal University,Nanchang 330022,China;National R&D Center for Freshwater Fish Processing,Nanchang 330022,China)

机构地区：[1]江西师范大学生命科学学院,江西南昌330022 [2]国家淡水鱼加工技术研发专业中心,江西南昌330022

出　　处：《食品工业科技》2025年第1期394-402,共9页Science and Technology of Food Industry

基　　金：国家自然科学基金(32260014,42407524);江西省自然科学基金(20224BAB215004,20224BAB215005)。

摘　　要：微生物菌株的改造优化是现代工业生物技术的核心,其中工业微生物底盘细胞的构建已成为合成生物学的研究热点之一。基于合成生物学“设计-构建-测试-学习”策略,对微生物底盘细胞理想设计和改造是优良细胞工厂构建的重要手段,本文对合成生物学和微生物底盘细胞的改造优化策略进行了相关概述,同时总结了基于合成生物学各工业微生物底盘细胞的构建及应用,最后对在构建底盘细胞时所遇到的障碍及未来的前景进行了展望,以推动具有优良性状的微生物细胞工厂的构建。The engineering and optimization of microbial strains form the core of modern industrial biotechnology,and the foundation of industrial microbial chassis cells has emerged as a hotspot in the field of synthetic biology.Guided by the"design-build-test-learn"approach,designing and engineering microbial chassis cells have become the critical strategy to develop excellent cell factories.This study presents a comprehensive review on strategies for modification and optimization of microbial chassis cells in synthetic biology.Meanwhile,this study summarized the applications and platforms benefited from industrial microbial chassis cells based on biology synthesis.Finally,this study discussed challenges encountered during chassis cell construction and put forward the future prospects to pave the way for microbial cell factories with superior attributes.

关 键 词：合成生物学 底盘细胞 基因组精简 工业微生物 微生物细胞工厂 

分 类 号：Q932[生物学—微生物学]