PEG-PLGA共载白藜芦醇纳米粒通过EMT和PI3K/Akt信号通路对结肠癌细胞的影响  

Effect of PEG-PLGA co-loaded resveratrol nanoparticles on colon cancer cells through EMT and PI3K/Akt signaling pathways

作　　者：高洪亮[1] 张浩 李明政 Gao Hongliang;Zhang Hao;Li Mingzheng(Department of Surgery,Tianjin Beichen Hospital,Tianjin 300400,China)

机构地区：[1]天津市北辰医院外科,天津300400

出　　处：《国际生物医学工程杂志》2024年第5期457-462,共6页International Journal of Biomedical Engineering

摘　　要：目的探讨聚乙二醇-聚乳酸羟基乙酸共聚物(PEG-PLGA)共载白藜芦醇纳米粒通过上皮-间质转化(EMT)和磷脂酰肌醇-3-激酶(PI3K)/蛋白激酶B(Akt)信号通路对结肠癌细胞的影响。方法采用人结肠癌HCT116细胞,随机分为对照组和实验组。实验组添加10μmol/L PEG-PLGA共载白藜芦醇纳米粒溶液50μl,对照组添加相同体积的培养基而不添加任何处理物质。使用MTT法测定细胞活力,流式细胞术分析细胞凋亡率,划痕实验检测细胞迁移能力;Western Blot分析凋亡、EMT和PI3K/Akt等相关信号通路蛋白的表达。结果与对照组相比,实验组的HCT116细胞存活率降低,细胞凋亡率升高(P<0.05)。与对照组相比,实验组HCT116细胞划痕宽度增加(P<0.001)。与对照组相比,实验组B淋巴细胞瘤-2(Bcl-2)蛋白表达下调(P<0.01),而Bcl-2相关X蛋白(Bax)的表达上调(P<0.05)。与对照组相比,实验组神经型钙黏附蛋白(N-cadherin)蛋白表达下调,而上皮型钙黏附蛋白(E-cadherin)的表达上调(P<0.01)。与对照组相比,实验组p-PI3K和p-Akt的水平均下调(均P<0.01)。结论PEG-PLGA共载白藜芦醇纳米粒可以降低结肠癌细胞的细胞活力、增殖能力和迁移能力,可能与抑制EMT和PI3K/Akt信号通路有关。Objective To investigate the effects of polyethylene glycol-poly(lactic-co-glycolic acid)(PEG-PLGA)co-loaded resveratrol nanoparticles on colon cancer cells through epithelial-mesenchymal transition(EMT)and phosphatidylinositol-3-kinase(PI3K)/protein kinase B(Akt)signaling pathways.Methods Human colon cancer HCT116 cells were randomly divided into the control group and the experimental group.The experimental group was supplemented with 50μl of 10μmol/L PEG-PLGA co-loaded resveratrol nanoparticle solution,and the control group was supplemented with the same volume of medium without adding any treatment substance.Cell viability was determined using the MTT assay,apoptosis rate was analyzed by flow cytometry,and cell migration ability was detected by a scratch test.Western Blot was used to analyze the expression of proteins of related signaling pathways such as apoptosis,EMT,and PI3K/Akt.Results Compared with the control group,the survival rate of colon cancer cells in the experimental group was reduced and the apoptosis rate was increased(P<0.05).Compared with the control group,the scratch width of HCT116 cells in the experimental group was greater(P<0.001).Compared with the control group,the expression of B-cell lymphoma-2(Bcl-2)protein in the experimental group was down-regulated(P<0.01),while the expression of Bcl-2 associated X protein(Bax)was up-regulated(P<0.05).Compared with the control group,the expression of N-cadherin protein in the experimental group was down-regulated,while the expression of E-cadherin was up-regulated(P<0.01).Compared with the control group,the levels of p-PI3K and p-Akt in the experimental group were down-regulated(both P<0.01).Conclusions PEG-PLGA co-loaded resveratrol nanoparticles can reduce the cell viability,proliferation,and migration of colon cancer cells,which may be related to the inhibition of EMT and PI3K/Akt signaling pathways.

关 键 词：白藜芦醇 结肠癌 纳米粒 上皮-间质转化 磷脂酰肌醇-3-激酶 蛋白激酶B 

分 类 号：R735.35[医药卫生—肿瘤]