β-elemene promotes miR-127-3p maturation,induces NSCLCs autophagy,and enhances macrophage M1 polarization through exosomal communication  

作　　者：Xiahui Wu Jie Wu Tingting Dai Qiangcheng Wang Shengjie Cai Xuehan Wei Jing Chen Ziyu Jiang 

机构地区：[1]Department of Oncology,Lianyungang Integrated Traditional Chinese and Western Medicine Clinical College,Nanjing University of Chinese Medicine,Nanjing,222002,China [2]Department of Oncology,The First People's Hospital of Lianyungang,Lianyungang,Jiangsu,222002,China [3]Department of Digestive System,Pukou Hospital of Traditional Chinese Medicine,Nanjing,210000,China [4]Department of Oncology,Jinling Hospital,School of Medicine,Nanjing University,Nanjing,210000,China [5]Department of Oncology,the Affiliated Cancer Hospital of Nanjing Medical University&Jiangsu Cancer Hospital&Jiangsu Institute of Cancer Research,Nanjing,210000,China [6]The Third Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing,210028,China [7]Department of Biochemistry and Molecular Biology,School of Medicine,Nanjing University of Chinese Medicine,Nanjing,210023,China

出　　处：《Journal of Pharmaceutical Analysis》2024年第9期1347-1360,共14页药物分析学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.:81973525);Traditional Chinese Medicine Development Project of Jiangsu Province(Grant No.:ZT202112).

摘　　要：β-elemene has been observed to exert inhibitory effects on a multitude of tumors,primarily through multiple pathways such as the inhibition of cancer cell proliferation and the induction of apoptosis.The present study is designed to elucidate the role and underlying mechanisms ofβ-elemene in the therapeutic intervention of non-small cell lung cancer(NSCLC).Both in vitro and in vivo experimental models corroborate the inhibitory potency ofβ-elemene on NSCLCs.Our findings indicate thatβ-elemene facilitates the maturation of miR-127-3p by inhibiting CBX8.Functioning as an upstream regulator of MAPK4,miR-127-3p deactivates the Akt/mTOR/p70S6K pathway by targeting MAPK4,thereby inducing autophagy in NSCLCs.Additionally,β-elemene augments the packaging of miR-127-3p into exosomes via SYNCRIP.Exosomal miR-127-3p further stimulates M1 polarization of macrophages by suppressing ZC3H4.Taken together,the detailed understanding of the mechanisms through whichβ-elemene induces autophagy in NSCLCs and facilitates M1 polarization of macrophages provides compelling scientific evidence supporting its potential utility in NSCLC treatment.

关 键 词：Β-ELEMENE miR-127-3p NSCLC Akt/mTOR/p70S6K MAPK4 

分 类 号：R730.5[医药卫生—肿瘤]