苹果酸舒尼替尼对裸鼠肾癌预后相关指标动态表达的影响  

Significance of the expression of prognostic related indicators of renal cancer in nude mice model

作　　者：蒋涛 刘辉[3] 陈姝 杨贤[2] 白淘[2] Jiang Tao;Liu Hui;Chen Shu;Yang Xian;Bai Tao(Department of Radiology,First Hospital of Shanxi Medical University,Taiyuan,030001,China;不详)

机构地区：[1]山西医科大学第一医院放射科,太原030001 [2]山西医科大学第一医院病理科,太原030001 [3]大同市第三人民医院麻醉科

出　　处：《山西医药杂志》2024年第23期1778-1782,共5页Shanxi Medical Journal

摘　　要：目的基于VHL-HIF-EPO信号通路,探讨苹果酸舒尼替尼早期干预对肾透明细胞癌预后的影响。方法本研究采取将肾细胞癌786-O细胞直接注射细胞悬液法注入裸鼠鼠背部皮下造模,设立不同给药时期3组和对照组。分别于干预后第5天、第20天、第35天和/或自然死亡后,通过苏木精-伊红(HE)染色和免疫组织化学法动态测定肿瘤组织中Ki67、增殖细胞核抗原(PCNA)、P53的表达情况,记录肿瘤体积、Fuhrman病理分级和生存时间。结果对各组之间第4次检测结果进行方差分析,各组Ki67阳性表达率差异有统计学意义(F=25.91,P<0.001);早期给药组,Ki67阳性表达率低。经Fisher′s法检验,各组之间血小板衍生生长因子受体(PDGFR)阳性表达率(χ^(2)=12.47,P=0.004),小鼠死亡率(χ^(2)=10.34,P=0.006)差异有统计学意义;早中期给药组PDGFR阳性表达率低,小鼠死亡率低。结论早期靶向药物干预可降低肿瘤Ki67、PDGFR阳性表达,并降低死亡率。因此,通过早期靶向药物干预可能提高肾细胞癌患者的预后,在实际工作中有必要进行早期干预。Objective Based on the VHL-HIF-EPO signaling pathway,we screened the possible prognostic factors through the intervention of significance on nude mice with clear cell renal cell carcinoma.Methods In this study,786-O cells were injected into nude mice subcutaneously into the back of nude miceto establish rat model.There were 3 groups which were set up according to the period of different drug delivery and one control group.The expressions of Ki67,PCNA and P53 in tumor tissues were detected by HE staining and immunohistochemistry for four times on the 5th,20th and 35th day after intervention and/or natural death,respectively.The expression of related indexes,tumor volume and Fuhrman pathological grade were measured dynamically and survival time was recorded.Results The results of the fourth test between the groups were compared by ANOVE,there were the statistically different in the positive expression rate of Ki67(F=25.91,P<0.001).In the early administration groups,the expression rate of Ki67 was the lowest.Meantime,by Fisher′s test,there were the statistically different in the positive expression of PDGFR(χ^(2)=12.47,P=0.004)and the mouse mortality(χ^(2)=10.34,P=0.006)among different groups.The positive expression of PDGFR and the mouse mortality in the early and middle administration groups was the lowest.Conclusion Early targeted drug intervention can reduce the positive expression of Ki67 and PDGFR,and delay the tumor growth.Therefore,intervention with early targeted drugs may improve the prognosis of RCC patients,and early intervention is necessary in practical work.

关 键 词：裸鼠 肾透明细胞癌 靶向治疗 预后 

分 类 号：R737.11[医药卫生—肿瘤]