高脂饮食联合持续光照对大鼠动脉硬化及心功能的影响及其可能机制  

COMBINED EFFECTS OF HIGH-FAT DIET AND CONTINUOUS ILLUMINATION ON ARTERIOSCLEROSIS AND CARDIAC FUNCTION IN RATS AND POSSIBLE MECHANISMS INVOLVED

作　　者：赵震宇[1] 张冬梅[2] 岳芳芝 杨达峰[3] 马静[3] 夏珂[3] ZHAO Zhen-yu;ZHANG Dong-mei;YUE Fang-zhi;YANG Da-feng;MA Jing;XIA Ke(Department of Clinical Pharmacology,Xiangya Hospital,Central South University,Changsha 410008,China;Department of Endocrinolog,Xiangya Hospital,Central South University,Changsha 410008,China;Department of Cardiology,Xiangya Hospital,Central South University,Changsha 410008,China)

机构地区：[1]中南大学湘雅医院临床药理研究所,长沙410008 [2]中南大学湘雅医院内分泌科,长沙410008 [3]中南大学湘雅医院心血管内科,长沙410008

出　　处：《营养学报》2024年第4期340-347,共8页Acta Nutrimenta Sinica

基　　金：湖南省科技重大专项基金(No.2021SK1020);湖南省自然科学基金(No.2023JJ30948);湖南省卫生健康委基金(No.202203014687)。

摘　　要：目的观察高脂饮食联合持续光照处理对SD大鼠动脉硬化相关血脂指标、主动脉硬化及心功能的影响,并探讨其可能机制。方法将32只SD雄性大鼠随机分为4组:A组:正常对照组(常规饮食+正常光照);B组:持续光照组(常规饮食+持续光照);C组:高脂饮食组(高脂饮食+正常光照);D组:高脂饮食联合持续光照组(高脂饮食+持续光照);每组各8只,记录SD大鼠体重,喂养12w后处死大鼠。比较各组SD大鼠体重、血糖(BS)、空腹胰岛素(Ins)、胰岛素抵抗指数(HOMA-IR)、传统血脂指标[总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)]、动脉硬化相关血脂指标[血浆致动脉硬化指数(AIP)、动脉粥样硬化指数(AI)、脂蛋白结合指数(LCI)、非高密度脂蛋白(non-HDL-C)、总胆固醇比高密度脂蛋白胆固醇(TC/HDL-C)、低密度脂蛋白比高密度脂蛋白胆固醇(LDL-C/HDL-C)],及血浆炎症因子指标[肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)]的差异;观察各组大鼠主动脉形态、主动脉前纤维蛋白-1(profilin-1)的蛋白与基因表达差异;观察各组大鼠的心功能指标[心率(HR)、每搏量(SV)、心输出量(CO)和左室射血分数(LVEF)、左室短轴缩短率(FS)]之间的差异。结果(1)高脂饮食联合持续光照组SD大鼠,其体重、Ins、HOMA-IR、BS、TC、LDL-C水平最高,HDL-C水平最低,其中:体重、Ins、HOMA-IR、TC均高于其它三组(均P<0.05),BS高于对照组和持续光照组(均P<0.05),LDL-C高于对照组(P<0.05),HDL-C低于对照组和持续光照组(均P<0.05)。(2)高脂饮食联合持续光照组SD大鼠,其AI、LCI、non-HDL-C、TC/HDL-C、LDL-C/HDL-C、TNF-α、IL-6水平均最高,其中:AI、non-HDL-C、TC/HDL-C、TNF-α均高于其它三组(均P<0.05),LCI、LDL-C/HDL-C、IL-6均高于对照组和持续光照组(均P<0.05),AIP高于对照组(P<0.05)。(3)主动脉形态及Profilin-1表达:高脂饮食联合持续光照组SD大鼠主动脉破坏程Objective To observe the effects of high-fat diet combined with continuous illumination on arteriosclerosisrelated blood lipid indexes,aortic sclerosis and cardiac function in SD rats,and explore its possible mechanisms.Methods Thirty-two SD male rats were randomly divided into 4 groups:Group A,control group(regular diet+normal illumination);Group B,continuous illumination group(regular diet+continuous illumination);Group C,high-fat diet group(high fat diet+normal illumination);Group D,high-fat diet combined with continuous illumination group(high-fat diet+continuous illumination).Rats were fed for 12 weeks.The differences in body weight,blood glucose(BS),fasting insulin(Ins),insulin resistance index(HOMA-IR),blood lipids[total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),triglyceride(TG)],arteriosclerosis-related blood lipid indexes[plasma arteriosclerosis index(AIP),atherogenic index(AI),lipoprotein combine index(LCI),non-high-density lipoprotein cholesterol(non-HDL-C),total cholesterol to high-density lipoprotein cholesterol(TC/HDL-C),low-density lipoprotein cholesterol to high-density lipoprotein cholesterol(LDL-C/HDL-C)],and plasma inflammatory factors[tumor necrosis factor-α(TNF-α),interleukin-6(IL)were compared.At the same time,the morphological changes of aortic sclerosis were observed,and the protein and gene expressions of profilin-1 in aorta were analyzed.Cardiac function indexes[heart rate(HR),stroke volume(SV),cardiac output(CO),left ventricular ejection fraction(LVEF)and left ventricular short axis shortening rate(LVFS)]were observed.Results(1)The rats fed with high-fat diet combined with continuous illumination had the highest levels of body weight,Ins,HOMA-IR,BS and TC,LDL-C,and the lowest level of HDL-C.Among them,the body weight,Ins,HOMA-IR and TC were all higher in group D than in the other three groups(all P<0.05),the BS was higher than the control and continuous illumination group(all P<0.05),the LDL-C was higher than the control gro

关 键 词：高脂饮食联合持续光照 动脉硬化相关血脂指标 主动脉硬化 心功能 SD大鼠 

分 类 号：R363.2[医药卫生—病理学]