颈外动脉免结扎法制备小鼠大脑中动脉栓塞模型的效果观察  

Observations of external carotid artery ligation free method in establishing the model of middle cerebral artery occlusion in mice

作　　者：马福浩 胡俊豪 穆秦颖 王浩 刘之荣 史明 MA Fuhao;HU Junhao;MU Qinying;WANG Hao;LIU Zhirong;SHI Ming(Department of Neurology,Xijing Hospital,Air Force Medical University,Xi’an 710032;Department of Neurology,987th Hospital of the PLA Joint Service Support Force,Baoji 721015,China)

机构地区：[1]空军军医大学西京医院神经内科,西安710032 [2]中国人民解放军联勤保障部队第987医院神经内科,宝鸡721015

出　　处：《神经解剖学杂志》2024年第5期558-564,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82071464)。

摘　　要：目的:为提高大脑中动脉栓塞(MCAO)模型的可操作性和稳定性,我们建立了一种新的MCAO模型制备方法——颈外动脉免结扎(ECA-LF)法,通过与传统Longa法和Koizumi法比较以明确其优势。方法:100只C57BL/6雄性小鼠,随机分为4组:Sham组、Koizumi组、Longa法和ECA-LF组,比较3种方法在手术操作时间、术后动物存活率、神经功能评分、梗死面积稳定性等方面的优劣。结果:ECA-LF组在模型制作时间上显著低于Longa组和Koizumi组;与Longa组相比,ECA-LF组Garcia评分更低,但与Koizumi组无显著差别;术后7 d,ECA-LF组小鼠的存活率明显高于其他两组。Morris水迷宫(MWM)实验表明,ECA-LF组在学习与记忆能力上与其他实验组差别不大。ECA-LF组的脑水肿程度及脑梗死面积与其它两组相当。结论:与传统Longa法和Koizumi法相比,ECA-LF法简化手术步骤、显著缩短了模型制作时间,提高了模型的存活率,在脑卒中实验研究中上具备一定的优势。Objective:To improve the operability and stability of the middle cerebral artery occlusion(MCAO) model,we established a novel method,external carotid artery ligation free(ECA-LF),for MCAO model and compared its advantages with the traditional Longa method and Koizumi method.Methods:A total of 100 male C57BL/6 mice were randomly divided into four groups:sham group,Koizumi group,Longa group,and ECA-LF group.The advantages and disadvantages of each method were evaluated in terms of surgical operation time,postoperative animal survival rate,postoperative animal survival rate,neurological functional score,and stability of infarct size.Results:The ECA-LF method spent less time than either the Longa or the Koizumi method during the course of making MCAO model.Compared with the Longa group,the ECA-LF group had a lower Garcia score,but was comparable to the Koizumi group.The survival rate of animals in the ECA-LF group was significantly higher than the other two groups 7 days after surgery.The Morris water maze(MWM) test showed that there was no significant difference between the ECA-LF group and other two groups in learning and memory ability.The degree of cerebral edema and infarct size in the ECA-LF group were comparable to the other two groups.Conclusion:Compared with the traditional Longa and Koizumi methods,the ECA-LF method simplifies the surgical procedures,shortens the model-making time,and improves the survival rate of model animal with similar stability to the traditional methods.It has certain advantages in stroke experimental research.

关 键 词：线栓法 大脑中动脉栓塞 小鼠 

分 类 号：R-332[医药卫生] R743.3