Advances in micropatterning technology for mechanotransduction research  

作　　者：Xinyu Hu Min Bao 

机构地区：[1]Institute for Molecules and Materials,Radboud University,Heyendaalseweg 135,6525 AJ,Nijmegen,the Netherlands [2]Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision and Brain Health),First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325001,Zhejiang,China

出　　处：《Mechanobiology in Medicine》2024年第3期31-39,共9页力学生物学与医学（英文）

基　　金：supported by start-up funding(grant OJQD2022015)from Oujiang Laboratory;the National Natural Science Foundation of China(grant 82301873).

摘　　要：Micropatterning is a sophisticated technique that precisely manipulates the spatial distribution of cell adhesion proteins on various substrates across multiple scales.This precise control over adhesive regions facilitates the manipulation of architectures and physical constraints for single or multiple cells.Furthermore,it allows for an indepth analysis of how chemical and physical properties influence cellular functionality.In this comprehensive review,we explore the current understanding of the impact of geometrical confinement on cellular functions across various dimensions,emphasizing the benefits of micropatterning in addressing fundamental biological queries.We advocate that utilizing directed self-organization via physical confinement and morphogen gradients on micropatterned surfaces represents an innovative approach to generating functional tissue and controlling morphogenesis in vitro.Integrating this technique with cutting-edge technologies,micropatterning presents a significant potential to bridge a crucial knowledge gap in understanding core biological processes.

关 键 词：MECHANOTRANSDUCTION MICROPATTERNING Organoid Cell geometry Cell differentiation Tissue engineering 

分 类 号：R318[医药卫生—生物医学工程]