非小细胞肺癌患者PRX1、CACNA2D1表达与调强放疗疗效的相关性研究  

Correlation between PRX1,CACNA2D1 expression and the efficacy of intensity-modulated radiotherapy in patients with non-small cell lung cancer

作　　者：王冠 李敏 张小妮 刘浩聪 WANG Guan;LI Min;ZHANG Xiaoni;LIU Haocong(Department of Radiotherapy,Shaanxi Cancer Hospital,Xi′an,Shaanxi 710065,China;Department of Oncology,Xi′an International Medical Center Hospital,Xi′an,Shaanxi 710100,China)

机构地区：[1]陕西省肿瘤医院放疗科,陕西西安710065 [2]西安国际医学中心医院肿瘤科,陕西西安710100

出　　处：《国际检验医学杂志》2024年第24期2945-2950,共6页International Journal of Laboratory Medicine

基　　金：陕西省自然科学基础研究项目(2022JQ-960)。

摘　　要：目的 分析非小细胞肺癌(NSCLC)患者硫氧还原蛋白过氧化物酶1(PRXl)、钙通道α2δ1亚基(CACNA2D1)表达与调强放疗效果的相关性。方法 选取2020年1月至2022年1月于陕西省肿瘤医院接受调强放疗的112例NSCLC患者的癌组织为NSCLC组,并根据放疗疗效,将112例NSCLC患者分为有效组(74例)和无效组(38例),以60例接受手术治疗的NSCLC患者的癌旁正常肺组织为对照组。采用免疫组织化学及蛋白免疫印迹检测组织中PRX1、CACNA2D1表达。采用Spearman秩相关分析PRX1与CACNA2D1表达的相关性。采用多因素Logistic回归分析NSCLC患者调强放疗疗效的影响因素。结果 NSCLC组PRX1、CACNA2D1阳性率高于对照组(χ^(2)=46.615、46.456,P<0.05)。NSCLC组PRX1、CACNA2D1相对灰度值高于对照组(t=9.357、9.509,P<0.05)。NSCLC癌组织PRX1与CACNA2D1表达呈正相关(r=0.724,P<0.001)。TNM分期Ⅲ~Ⅳ期、低分化程度、淋巴结转移患者癌组织PRX1、CACNA2D1阳性率均高于TNM分期Ⅰ~Ⅱ期、中高分化、无淋巴结转移患者(P<0.05)。无效组TNM分期Ⅲ~Ⅳ期占比、淋巴结转移占比、PRX1阳性率、CACNA2D1阳性率均高于有效组(P<0.05)。TNM分期Ⅲ~Ⅳ期、淋巴结转移、PRX1阳性、CACNA2D1阳性是影响NSCLC患者调强放疗疗效的危险因素(P<0.05)。结论 NSCLC患者PRX1、CACNA2D1阳性率增加,与TNM分期、分化程度及淋巴结转移有关,均是影响NSCLC患者调强放疗疗效的危险因素。Objective To analyze the correlation between Peroxiredoxin 1(PRXl)and Calcium channelα2δ1 subunit(CACNA2D1)expression and the efficacy of intensity-modulated radiotherapy in patients with non-small cell lung cancer(NSCLC).Methods The cancer tissues in totally 112 NSCLC patients who received intensity-modulated radiotherapy in Shaanxi Cancer Hospital from January 2020 to January 2022 were selected as the NSCLC group,and according to the efficacy of radiotherapy,112 NSCLC patients were divided into an effective group(74 cases)and an ineffective group(38 cases).Normal lung tissues adjacent to cancer in 60 NSCLC patients undergoing surgical treatment were selected as the control group.Immunohistochemistry and Western blot were used to detect the expression of PRX1 and CACNA2D1 in tissues.Spearman rank correlation analysis was used to investigate the correlation between PRX1 and CACNA2D1 expression.Multivariate Logistic regression was used to analyze the influencing factors of efficacy of intensity-modulated radiotherapy in NSCLC patients.Results The positive rates of PRX1 and CACNA2D1 in NSCLC group were higher than those in control group(χ^(2)=46.615,46.456,P<0.05).The relative grayscale values of PRX1 and CACNA2D1 in NSCLC group were higher than those in control group(t=9.357,9.509,P<0.05).The expression of PRX1 and CACNA2D1 in NSCLC cancer tissues was positively correlated(r=0.724,P<0.001).The positivity rates of PRX1 and CACNA2D1 in cancer tissues of patients with TNM stageⅢ-Ⅳ,low differentiation,and lymph node metastasis were higher than those of patients with TNM stageⅠ-Ⅱ,medium and high differentiation,and no lymph node metastasis(P<0.05).The proportion of patients with TNM stageⅢ-Ⅳ,the proportion of patients with lymph node metastasis,positivity rate of PRX1 and positivity rate of CACNA2D1 in the ineffective group were higher than those in the effective group(P<0.05).TNM staging stagesⅢ-Ⅳ,lymph node metastasis,PRX1 positive,and CACNA2D1 positive were risk factors affecting the efficacy of i

关 键 词：非小细胞肺癌 硫氧还原蛋白过氧化物酶1 钙通道α2δ1亚基 调强放疗 

分 类 号：R734.2[医药卫生—肿瘤]