海藻酸钠寡糖硫酸化衍生物的制备及其抗氧化活性  

Preparation and Antioxidant Activity of Sulfated Derivatives of Alginate Oligosaccharides

作　　者：王康康 王柏翔 孙昊鉴 李岩峰 吴海歌[1,2,3] 姚子昂 WANG Kangkang;WANG Baixiang;SUN Haojian;LI Yanfeng;WU Haige;YAO Zi'ang(College of Life and Health,Dalian University,Dalian 116600,Liaoning,China;Liaoning Province Marine Drug Research and Development Professional Technology Innovation Center,Dalian 116600,Liaoning,China;Dalian Key Laboratory of Marine Biotechnology,Dalian 116600,Liaoning,China;College of Life Science,Dalian Minzu University,Dalian 116600,Liaoning,China)

机构地区：[1]大连大学生命健康学院,辽宁大连116600 [2]辽宁省海洋药物研发专业技术创新中心,辽宁大连116600 [3]大连市海洋生物技术重点实验室,辽宁大连116600 [4]大连民族大学生命科学学院,辽宁大连116600

出　　处：《食品研究与开发》2024年第24期119-128,共10页Food Research and Development

摘　　要：以海藻酸钠为原料,经过氧化法降解、硫酸化修饰得到硫酸化海藻酸钠寡糖。选择氯磺酸‐吡啶体积比、硫酸化试剂‐海藻酸钠寡糖溶液体积比、反应温度和反应时间为单因素,以海藻酸钠寡糖(alginate oligosaccharides,AOS)硫酸化修饰取代度为评价指标,通过响应面试验对氯磺酸‐吡啶法制备硫酸化海藻酸钠寡糖进行优化。确定最佳反应条件为氯磺酸‐吡啶体积比为1∶7,硫酸化试剂与海藻酸钠寡糖溶液体积比为1∶3,反应时间3h,反应温度40℃。在此条件下制备3种不同取代度的硫酸化海藻酸钠寡糖,进行体外抗氧化活性测定。体外抗氧化活性结果表明,硫酸化修饰对AOS的体外抗氧化活性有较大影响,修饰后可以提高AOS的还原力和清除ABTS+自由基、DPPH自由基的能力,且能力随取代度增高而增强。The sulfated alginate oligosaccharides(AOS)were obtained from sodium alginate through oxidative degradation and sulfation modification.The volume ratio of chlorosulfonic acid to pyridine,the volume ratio of sulfating reagent to AOS solution,reaction temperature,and reaction time were selected as single factors.The degree of sulfation substitution of AOS was used as the evaluation index.Response surface methodology was employed to optimize the chlorosulfonic acid‐pyridine method for preparing sulfated AOS.The optimal reaction conditions were determined to be a chlorosulfonic acid‐pyridine volume ratio of 1∶7,a sulfating reagent to AOS solution volume ratio of 1∶3,a reaction time of 3 h,and a reaction temperature of 40℃.Under these conditions,three types of sulfated AOS with different degrees of substitution were prepared and their in vitro antioxidant ac‐tivities were measured.The results of the in vitro antioxidant activity tests indicated that sulfation modification significantly enhanced the antioxidant activity of AOS.The modification improved the reducing power and the ability to scavenge ABTS+and DPPH free radicals,with the effects increasing with higher degrees of substitution.

关 键 词：海藻酸钠寡糖 硫酸化 氯磺酸‐吡啶 条件优化 抗氧化性 

分 类 号：TQ281[化学工程—有机化工]