草苁蓉纳拉苷对SMMC-7721肝癌细胞上皮-间质转化的抑制作用  

Boschniakia rossica boschnaloside inhibition of epithelial-mesenchymal transition of hepatoma SMMC-7721 cells

作　　者：金爱花 朱洁波 全吉淑[2] JIN Aihua;ZHU Jiebo;QUAN Jishu(the Affiliated Hospital of Yanbian University(Yanbian Hospital),Yanji 133000;China.2.Yanbian University Medical College,Yanji 133002)

机构地区：[1]延边大学附属医院(延边医院),吉林延吉133000 [2]延边大学医学院,吉林延吉133002

出　　处：《中国比较医学杂志》2024年第11期11-18,共8页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(82060113)。

摘　　要：目的探讨草苁蓉纳拉苷(Boschniakia rossica boschnaloside,BRBN)对SMMC-7721肝癌细胞上皮-间质转化(epithelial-mesenchymal transition,EMT)以及侵袭、迁移的抑制作用。方法用转化生长因子β1(transforming growth factorβ1,TGF-β1)诱导SMMC-7721肝癌细胞建立EMT模型。将其分为对照组、模型组、BRBN组,利用细胞划痕愈合实验检测肝癌细胞迁移能力,Transwell侵袭实验检测肝癌细胞侵袭能力,Western blot法检测E-钙黏蛋白、N-钙黏蛋白、波形蛋白、Slug、Twist1、ZEB1及信号转导和转录激活因子3(signal transducers and activators of transcription 3,STAT3)的蛋白表达。结果TGF-β1使SMMC-7721肝癌细胞发生纤维状间质样改变,E-钙黏蛋白表达下调,N-钙黏蛋白和波形蛋白表达上调,提示SMMC-7721细胞获得间质表型。与模型组比较,BRBN组SMMC-7721细胞侵袭率和迁移率均显著降低(P<0.05),E-钙黏蛋白表达显著升高(P<0.05),N-钙黏蛋白和波形蛋白表达显著降低(P<0.05),Slug、Twist1、ZEB1蛋白表达均显著下调(P<0.05),STAT3磷酸化水平显著降低。结论BRBN可抑制SMMC-7721肝癌细胞侵袭、迁移,其作用机制可能与STAT3介导的EMT逆转有关。Objective To investigate the inhibitory effect of Boschniakia rossica boschnaloside(BRBN)on the epithelial-mesenchymal transition(EMT),invasion,and migration of human hepatoma SMMC-7721 cells.Methods Transforming growth factorβ1(TGF-β1)was used to induce an EMT model of SMMC-7721 cells.The cells were divided into a control group,model group,and BRBN group.Cell migration was detected with a wound healing test,and cell invasion was observed by Transwell chamber assay.The expression of E-cadherin,N-cadherin,Vimentin,Slug,Twist1,ZEB1,and signal transducers and activators of transcription 3(STAT3)were determined with the Western blot method.Results TGF-β1 resulted in spindle-shaped SMMC-7721 cells,down-regulated the expression of E-cadherin,and upregulated the expression of N-cadherin and vimentin,suggesting that SMMC-7721 cells obtained a mesenchymal phenotype.Compared with the model group,the BRBN group SMMC-7721 cells’expression of E-cadherin was significantly increased,their expression of N-cadherin and vimentin were reduced,and their expression of Slug,Twist1,and ZEB1,as well as STAT3 phosphorylation,were down-regulated.Conclusions BRBN inhibits the invasion and migration of human hepatoma SMMC-7721 cells,possibly by reversing EMT through the STAT3 pathway.

关 键 词：草苁蓉 草苁蓉纳拉苷 SMMC-7721细胞 上皮-间质转化 STAT3 

分 类 号：R-33[医药卫生]