应用三维时间飞跃法磁共振血管成像(3D-TOF-MRA)技术观察长爪沙鼠脑动脉的方法初探  

Preliminary study of three-dimensional time-of-flight magnetic resonance angiography for observing cerebral arteries in Mongolian gerbils

作　　者：焦昆 张静 孟霞 王战京 雷建锋 陈柏安 卢静 JIAO Kun;ZHANG Jing;MENG Xia;WANG Zhanjing;LEI Jianfeng;CHEN Baian;LU Jing(Department of Laboratory Animal,Capital Medical University,Beijing 100069,China;Department of Experimental Zoology,School of Basic Medicine,Capital Medical University,Beijing 100069;Core Facility Center,Capital Medical University,Beijing 100069)

机构地区：[1]首都医科大学实验动物部,北京100069 [2]首都医科大学基础医学院实验动物学学系,北京100069 [3]首都医科大学中心实验室,北京100069

出　　处：《中国比较医学杂志》2024年第11期68-74,共7页Chinese Journal of Comparative Medicine

基　　金：国家重点研发计划(2021YFF0702805)。

摘　　要：目的探究通过3D-TOF-MRA方法确认长爪沙鼠实验前条件的可行性。方法使用7.0 T MRI扫描仪对长爪沙鼠脑动脉进行扫描,扫描数据采用RadiAnt DICOM Viewer软件进行后续处理;采用乳胶灌注法对影像学结果进行验证与比较;通过MIMICS软件对长爪沙鼠及大鼠的后交通支及周围脑血管进行三维重建;使用此方法筛选血管发育异常的长爪沙鼠个体验证本方法的应用效果。结果3D-TOF-MRA技术可以有效地观察到活体长爪沙鼠脑动脉;3D-TOF-MRA技术观察长爪沙鼠脑主要动脉血管的准确性较高,并且能观察组织内部血管,但对于较细血管分支的观察效果不如乳胶灌注法;3D-TOF-MRA技术所得数据可以用于血管的三维重建;3D-TOF-MRA技术应用于长爪沙鼠脑动脉结构异常个体的筛选具有较好效果。结论3D-TOF-MRA技术可以应用于活体长爪沙鼠脑动脉的结构观察及相关研究。Objective To explore the feasibility of confirming pre experimental conditions in Mongolian gerbils using three-dimensional time-of-flight magnetic resonance angiography(3D-TOF-MRA).Methods The cerebral arteries in Monglian gerbils were imaged using a 7.0 T magnetic resonance imaging scanner and the data were processed using RadiAnt DICOM Viewer software.The imaging result were then validated and compared using the latex perfusion method,and 3D reconstruction of the posterior communicating branch and surrounding cerebral blood vessels were performed using analytical software.Finally,we attempted to use this method to screen for abnormal vascular development in Mongolian gerbils,to verify the effectiveness of this method.Results 3D-TOF-MRA effectively demonstrated the cerebral arteries in live Mongolian gerbils.The technique showed high accuracy for observing the main cerebral arteries in Mongolian gerbils,but its ability to show finer vascular branches was not as good as the latex perfusion method.The data obtained using 3D-TOF-MRA could be used for 3D reconstruction of blood vessels,and the technology could be applied to screen for abnormal arterial structures in Mongolian gerbils.Conclusions 3D-TOF-MRA technology can be applied for the structural observation and related research of the cerebral arteries in live Mongolian gerbils.

关 键 词：核磁共振 血管成像 长爪沙鼠 脑 动脉 

分 类 号：R-33[医药卫生]