Encapsulation of Clove Oil Nanoemlusion in Chitosan-Based Nano-Composite: In Vitro and in Vivo Antifungal Activity against Rhizoctonia solani and Sclerotium rolfsii  

作　　者：Ahmed Mahmoud Ismail Eman Said Elshewy Isra HAli Naglaa Abd Elbaki Sallam Muhanna Eman Yehia Khafagi 

机构地区：[1]Department of Arid Land Agriculture,College of Agricultural and Food Sciences,King Faisal University,Al-Ahsa,31982,Saudi Arabia [2]Pests and Plant Diseases Unit,College of Agricultural and Food Sciences,King Faisal University,Al-Ahsa,31982,Saudi Arabia [3]Vegetable Diseases Research Department,Plant Pathology Research Institute,Agricultural Research Center(ARC),Giza,12619,Egypt [4]Department of Pharmaceutics,Faculty of Pharmacy,University of Sadat City,Sadat City,32958,Egypt [5]Nanomedicine Laboratory,Faculty of Pharmacy,University of Sadat City,Sadat City,32958,Egypt

出　　处：《Phyton-International Journal of Experimental Botany》2024年第11期2787-2811,共25页国际实验植物学杂志（英文）

基　　金：This research was kindly funded by the Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research,King Faisal University,Saudi Arabia,grant number(KFU241802).

摘　　要：Rhizoctonia solani Kühn and Sclerotium rolfsii Sacc.are the primary soil-borne plant diseases responsible for sig-nificant reductions in global crop yields.The primary goal of this study was to investigate the antifungal potentials of clove essential oil(CEO),nanoemulsion form(CEONE)and chitosan/nanoemulsion nanocomposite(CS/CEONE)against R.solani and S.rolfsii through in vitro and in vivo trials.Both CEONE and CS/CEONE were prepared and investigated for their physical chemical and morphological characterization.The poisoned medium method was utilized to evaluate the inhibitory effects of CEO,CEONE and CS/CEONE on the mycelial growth and enzymatic activity of R.solani and S.rolfsii.The changes of hyphae of R.solani and S.rolfsii in response to treating with CEONE and CS/CEONE were observed with scanning electron microscope(SEM).The results revealed that CEONE have larger size 86±3 nm and a broader range of PDI 0.121±0.011 on the average.While,CS/CEONE has smaller size of 49±4 nm and narrower PDI of 0.099±0.009.Both nanoemulsions had uniform spherical nanodroplets form and exhibited acidic nature.Fourier Transform Infrared Spectroscopy(FTIR)and UV-Visible Spectroscopy(UV-Vis)verified the successful incorporation of both CS and CEO within the nanoe-mulsion system.The results demonstrated a sustained and prolonged release profile from CS/CEONE for up to 4 days.The inhibitory effect of CEONE and CS/CEONE showed dose-dependent activity against mycelial growth of both fungi.CEONE and CS/CEONE at concentration 500µL/L exhibited the strongest inhibition with a sig-nificant(p<0.05)variation among them with value ranging from 56.11%to 71.94%and 52.2%to 79.2%,respec-tively.Comparing to control,CS/CEONE revealed the highest inhibitory effect against S.rolfsii after 96 h followed by CEONE with value reached 50.6%and 44.1%,respectively.The antifungal activity of the nanoemulsion showed strain–dependent behavior,where S.rolfsii was the most affected.SEM images showed changes in the hyphal structure of S.rolfsii and

关 键 词：Black scurf chitosan clove oil DAMPING-OFF INHIBITORY nanocomposite NANOEMULSION stem canker 

分 类 号：Q94[生物学—植物学]