Response of the Ginseng C_(2)H_(2)-Type Zinc Finger Protein Family PgZFPs Gene to Methyl Jasmonate Regulation  

作　　者：Yue Jiang Lingyu Liu Kangyu Wang Mingzhu Zhao Ping Chen Jun Lei Yanfang Wang Meiping Zhang Yi Wang Guang Chen 

机构地区：[1]College of Life Science,Jilin Agricultural University,Changchun,130118,China [2]Research Center for Ginseng Genetic Resources Development and Utilization,Jilin Province,Jilin Agricultural University,Changchun,130118,China [3]College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun,130118,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第11期3055-3071,共17页国际实验植物学杂志（英文）

基　　金：This work was supported by the Department of Science and Technology of Jilin Province(20240101227JC,20210402043GH,20200801063GH,20190201264JC,20190103104JH,20180414077GH,and 20180101027JC);the Development and Reform Commission of Jilin Province(2016C064 and 2018C047-3).

摘　　要：The main active components of ginseng are ginsenosides,which play significant roles in treating cardiovascular diseases,cancer,and providing antioxidant effects.Ginsenosides are primarily synthesized through the mevalo-nate pathway and the methylerythritol phosphate pathway.Many key enzyme genes involved in this biosynthetic process have been cloned and validated,yet the regulatory functions of transcription factors remain unclear.The C_(2)H_(2)-type zincfinger protein family,one of the largest families of transcription factors,is crucial in plant growth and development,response to biotic and abiotic stresses,and regulation of secondary metabolism.This study,based on the ginseng transcriptome database from Jilin,conducted a correlation analysis between the expression levels of PgZFPs genes in the Jilin ginseng C_(2)H_(2)-type zincfinger protein family and ginsenoside content,a gen-ome-wide association study of PgZFPs,and co-expression analysis of PgZFPs with validated key enzyme genes.Ultimately,five candidate genes involved in ginsenoside biosynthesis were identified.The involvement of PgZFP27 and PgZFP-59-02 genes from the PgZFPs family in the biosynthesis of ginsenosides was validated through in vitro methyl jasmonate(MeJA)induction experiments.This result provides new genetic resources for the biosynthesis of ginsenosides.

关 键 词：GINSENOSIDES C_(2)H_(2)-type zincfinger protein family MeJA induction functional study SIT SITIENS FLC FLACCA MVA Mevalonate MEP 2-C-methyl-D-erythritol 4-phosphate GWAS Genome-wide association studies HPLC High-performance liquid chromatography 

分 类 号：Q94[生物学—植物学]