脂肪酶试剂携带的污染对血清总胆汁酸水平检验的干扰作用分析  

An Analysis of the Interference Effect of Contamination Carried byLipase Reagent on Serum Total Bile Acid Level Detection

作　　者：王萌 翟燕红[1] 曹正 WANG Meng;ZHAI Yanhong;CAO Zheng(Department of Laboratory Medicine,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing Maternal and Child Health Care Hospital,Beijing 100026,China)

机构地区：[1]首都医科大学附属北京妇产医院/北京妇幼保健院检验科,北京100026

出　　处：《标记免疫分析与临床》2024年第11期2062-2065,共4页Labeled Immunoassays and Clinical Medicine

基　　金：北京市卫生健康科技成果和适宜技术推广项目(编号:BHTPP2022017)。

摘　　要：目的分析临床生化检测工作中脂肪酶(LPS)试剂携带的污染对于血清总胆汁酸(TBA)水平检测结果的影响。方法采用循环酶法检测不同样本中血清TBA水平,观察LPS的R1和R2试剂对于血清TBA检测的携带污染。结果LPS的R1和R2检测试剂均对血清TBA水平的检测存在显著的干扰(P<0.05),且这种干扰作用呈现递减趋势。结论血清TBA水平检测易受到LPS检测试剂的干扰。在临床检测工作中,检测人员可通过将互相干扰的项目设置在不同的检测模块中,从而保证检测水平的可靠性和准确度。Objective To analyze the interference effect of contamination carried by lipase(LPS)on the detection of serum total bile acid(TBA)levels in clinical biochemical tests.Methods Circulating enzyme method was applied to detect TBA levels in different serum samples,and the contaminations of LPS R1 and R2 reagents for serum TBA detection were also observed.Results Both LPS R1 and R2 detection reagents showed significant interference effect on the detection results of serum TBA levels(all P<0.05),and this interference effect displayed a decreasing trend.Conclusion There is contamination carried by LPS reagents,which could affect the accuracy of the serum TBA detection results.Clinical testing personnel can set items that interfere with each other in different testing modules to ensure the reliability and accuracy of the results.

关 键 词：脂肪酶 总胆汁酸 携带污染 生化检测 

分 类 号：R446.1[医药卫生—诊断学]