外源谷胱甘肽处理对青花菜幼苗硫代葡萄糖苷代谢的影响  

Effects of glutathione treatment on the metabolism of glucosinolates in broccoli seedlings

作　　者：赵凯文 马超超 李慕紫 邹宜芯 马鹤桐 李慧 Zhao Kaiwen;Ma Chaochao;Li Muzi;Zou Yixin;Ma Hetong;Li Hui(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院园艺园林学院,天津300392

出　　处：《天津农学院学报》2024年第6期28-33,共6页Journal of Tianjin Agricultural University

基　　金：天津市大学生创新训练计划项目(202210061104);天津市科技计划种业科技重大专项(18ZXZYNC00160)。

摘　　要：为探究外源谷胱甘肽(GSH)对硫代葡萄糖苷代谢的影响,本文以青花菜幼苗为研究对象,对其进行外施25 mg/L GSH处理,进而对不同处理时间青花菜幼苗中的总硫苷、萝卜硫素含量及黑芥子酶活性进行测定,对硫代葡萄糖苷代谢相关基因bolMAM1、bolUGT74B1、bolMYB28、bolMyr和bolPEN2的转录表达特征进行评价。结果表明,青花菜幼苗在外源GSH处理12 h时,其硫代葡萄糖苷含量显著高于对照。与之一致,硫代葡萄糖苷合成关键基因bolMAM1、bolUGT74B1转录表达水平也显著高于对照,bolMYB28转录表达水平虽然高于对照,但是未达到显著水平。进一步分析发现,黑芥子酶基因bolMyr和bolPEN2的表达水平及黑芥子酶活性在外源GSH处理24 h时达到最大值,并显著高于对照组。伴随着黑芥子酶活性升高,在外源GSH处理36 h时,处理组萝卜硫素的含量达到最高值,并显著高于对照组。综上所述,外施GSH对青花菜幼苗硫代葡萄糖苷合成及生物活性物质萝卜硫素生成具有积极效应。研究结果为采用外源物处理方法提高青花菜等十字花科植物中硫代葡萄糖苷,特别是其水解产物萝卜硫素的含量提供了重要数据支撑。In order to investigate the effects of exogenous glutathione(GSH)on the metabolism of glucosinolates,brocolli seedlings treated with 25 mg/L GSH were taken as experiment materials.The contents of glucosinolates and sulforaphane and the activity of myrosinase were detected,and the transcriptional expression characteristics of the genes related to glucosinolate metabolism in brocolli seedlings,including bolMAM1,bolUGT74B1,bolMYB28,bolMyr,and bolPEN2 were evaluated at different time points after GSH treatment.The results showed that at 12 h of exogenous GSH treatment,the content of glucosinolate in brocolli seedlings was significantly higher than that in the control.Consistent with this,the transcriptional expression levels of key genes for glucosinolates synthesis,bolMAM1 and bolUGT74B1 were significantly higher than those in the control.Although the transcriptional expression level of bolMYB28 was higher than that in the control,it did not reach a significant level.Further analysis revealed that the expression levels of the myrosinase genes bolMyr and bolPEN2,as well as the myrosinase activity,reached their maximum at 24 h of exogenous GSH treatment and were significantly higher than those in the control.With the increase of myrosinase activity,after 36 h of exogenous GSH treatment,the content of sulforaphane in the treatment group reached its highest value and was significantly higher than that in the control group.In summary,exogenous application of GSH has a positive effect on the synthesis of glucosinolates and the generation of the bioactive substance sulforaphane in brocolli seedlings.The research results provide important data support for the use of exogenous treatment methods to increase the content of glucosinolates,especially its hydrolysis product sulforaphane,in cruciferous plants such as broccoli.

关 键 词：青花菜 谷胱甘肽 硫代葡萄糖苷 萝卜硫素 黑芥子酶 

分 类 号：S635.9[农业科学—蔬菜学]