基于蛋白组学分析不同产地枸杞黄酮类化合物的差异  

The Difference of Biosynthesis of Flavonoids in Goji Berry from Different Regions Based on Proteomic Analysis

作　　者：孙霞芝 马瑞雪 杨超 范艳丽[1] Sun Xiazhi;Ma Ruixue;Yang Chao;Fan Yanli(College of Food Science and Engineering,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学食品科学与工程学院,银川750021

出　　处：《中国食品学报》2024年第11期348-358,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(32160534)。

摘　　要：目的:从蛋白质组学的角度解析不同产地“宁杞1号”枸杞黄酮类化合物的差异。方法:以宁夏、青海和甘肃成熟期“宁杞1号”枸杞果实为研究对象,利用串联质谱标签(TMT)结合液相色谱-串联质谱联用(LC-MS/MS)技术,探究不同产地枸杞的蛋白差异。结果:3个比较组共鉴定出716个差异表达蛋白(DEPs),KEGG通路富集共发现4条与黄酮类化合物相关的代谢途径,其中苯丙烷生物合成途径在3个比较组中均被显著富集(P<0.05),表明苯丙烷生物合成途径在枸杞黄酮合成中可能做出较大贡献。筛选出24个与黄酮合成相关的关键差异表达蛋白,发现苯丙氨酸解氨酶(PAL)、肉桂酸4-羟化酶(C4H)、查尔酮异构酶(CHI)、查尔酮合酶(CHS)等可能是宁夏、青海和甘肃枸杞黄酮类化合物生物合成的重要DEPs。结论:从蛋白质层面鉴定出与枸杞黄酮类化合物生物合成相关的差异蛋白,初步推测枸杞黄酮类化合物合成代谢通路,为探究不同产地枸杞黄酮生物合成差异奠定基础。Objective:To analyze the differences of goji flavonoids from different origins from the perspective of proteomics.Methods:The protein differences of goji berries from different origins were explored by tandem mass tag(TMT)combined with liquid chromatography-tandem mass spectrometry(LC-MS/MS)technology in Ningxia,Qinghai and Gansu.Results:A total of 716 differential expressed proteins(DEPs),and a total of four metabolic pathways associated with flavonoids were found in the KEGG pathway,and the phenylpropane biosynthesis pathway was significantly enriched in all three comparison groups(P<0.05),indicating that the phenylpropane biosynthesis pathway might make a greater contribution to the synthesis of goji flavonoids.Further screening of 24 key differentially expressed proteins related to flavonoid synthesis,it was found that phenylalanine ammonia-lyase(PAL),cinnamic acid 4-hydroxylase(C4H),chalcone isomerase(CHI),chalcone synthase(CHS),etc.might be important DEPs for the biosynthesis of flavonoids in Ningxia,Qinghai and Gansu.Conclusion:The differential proteins related to the biosynthesis of goji flavonoids were identified from the protein level,and the anabolic pathway of goji flavonoids was preliminarily speculated,which laid a foundation for exploring the differences in the biosynthesis of goji flavonoids in different origins.

关 键 词：枸杞 蛋白质组学 黄酮类化合物 关键差异表达蛋白 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]