接种比对微量元素促进厌氧消化体系产甲烷的影响  

The effect of inoculum/substrate on methane production in anaerobic digestion systems promoted by trace elements

作　　者：尹赵 孙辰 曹卫星 胡长伟 Yin Zhao;Sun Chen;Cao Weixing;Hu Changwei(Faculty of Science,Zhejiang Sci-Tech University,Hangzhou 310018,China;College of Biological,Chemical Science and Engineering,Jiaxing University,Jiaxing 314001,China)

机构地区：[1]浙江理工大学理学院,浙江杭州310018 [2]嘉兴学院生物与化学工程学院,浙江嘉兴314001

出　　处：《可再生能源》2024年第12期1570-1577,共8页Renewable Energy Resources

基　　金：国家重点研发计划项目(2021YFE0104600);国家自然科学基金青年基金项目(31901407)。

摘　　要：有关微量元素(TEs)在厌氧消化系统中的关键调控作用已经明确,但其内在作用规律和机理的解析还不够全面。文章通过研究厌氧消化体系中不同接种比(I/S)下TEs的变化规律,探究TEs对厌氧发酵的生物有效性,明确TEs化学形态与厌氧发酵过程中关键影响因子及微生物群落之间的相互作用关系。研究结果表明:在10批次的葡萄糖厌氧消化试验中,在I/S为1.5的条件下添加TEs后,甲烷产量提升了80.7%;在添加的3种TEs(Fe,Co和Ni)中,Fe元素的不同化学形态与厌氧消化中pH值、碱度、挥发性脂肪酸和氨氮含量的相关性最强;pH值与酸可挥发性硫化物含量是厌氧消化中TEs生物有效性的关键影响因子;pH值与Fe的4种化学形态均有明显相关性;厌氧消化体系稳定运行时,甲烷丝菌属(Methanosaeta)的相对丰度最高(53.8%),厌氧消化体系运行异常时,甲烷八叠球菌属(Methanosarcina)的相对丰度最高(34%)。The key regulatory role of trace elements(TEs)in anaerobic digestion systems has been established,but their intrinsic effects and mechanisms have not yet been fully elucidated.This study investigates the biological effects of TEs on biogas fermentation by examining the dynamic distribution of TEs under different inoculum/substrate(I/S)conditions in an anaerobic digestion system.It aims to clarify the interaction between the chemical form of TEs and the key influencing factors and microbial communities in the anaerobic fermentation process,Results from the 10 batches of experimental anaerobicdigestion of glucose showed an 80.7%enhancement in cumulative methane production with the addition of TEs at an I/S ratio of 1.5.Among the three TEs(Fe,Co and Ni)added,the different chemical forms of elemental Fe showed the strongest correlation with pH,alkalinity,volatile fatty acids and ammonia nitrogen content in anaerobic digestion.The pH value was notably correlated with the four chemical forms of Fe.When the anaerobic digestion system operates stably,the relative abundance of Methanosaeta was the highest(53.8%),while the relative abundance of Methanosarcina was the highest(34.0%)when the anaerobic digestion system operates abnormally.

关 键 词：厌氧消化 微量元素 化学形态 PH值 微生物多样性 

分 类 号：TK6[动力工程及工程热物理—生物能]