三氧化二砷对三阴乳腺癌干细胞MDA-MB-231细胞株凋亡和自噬作用机制研究  

Mechanism of Arsenic Trioxide on Apoptosis and Autophagy in Triple-negative Breast Cancer Stem Cell MDA-MB-231 Cell Line

作　　者：武瑞仙 李莹 陈云婷 庄文馨 洪日 WU Rui-xian;LI Ying;CHEN Yun-ting;ZHUANG Wen-xin;HONG Ri(Department of Breast Surgery,Shanghai Children's Medical Center Hainan Hospital,Shanghai Jiao Tong University School of Medicine,Sanya,Hainan,572000,China)

机构地区：[1]上海交通大学医学院附属上海儿童医学中心海南医院乳腺科,海南三亚572000

出　　处：《现代生物医学进展》2024年第23期4414-4416,共3页Progress in Modern Biomedicine

基　　金：上海交通大学医学院附属上海儿童医学中心海南医院金椰种子基金项目(JYZZ-201915);海南省自然科学基金青年基金项目(821QN1009);海南省卫生健康行业科研项目(20A200121)。

摘　　要：目的:探讨三氧化二砷对TNBC干细胞MDA-MB-231细胞株凋亡和自噬作用机制。方法:取第2代培养5天的干细胞进行实验,共分为4组:对照组、三氧化二砷作用组、Rapa联合三氧化二砷组和3-MA联合三氧化二砷组。观察检测细胞凋亡率、细胞自噬、蛋白表达情况。结果:三氧化二砷作用48 h,细胞总凋亡率为40%,采用3MA抑制细胞自噬活性,三氧化二砷诱导的细胞凋亡率降低约50%,Rapa可促使三氧化二砷诱导细胞凋亡增高约50%。与对照组相比,三氧化二砷组、Rapa联合三氧化二砷组、3-MA联合三氧化二砷组胞内荧光强度均较高。3-MA联合三氧化二砷组Bax、Beclin-1、P62蛋白表达高于三氧化二砷组、Rapa联合三氧化二砷组,Bcl-2蛋白表达低于三氧化二砷组、Rapa联合三氧化二砷组(P<0.05)。结论:三氧化二砷诱导的MDA-MB-231干细胞中自噬和细胞调亡现象共存且可能受共同基因的调控,三氧化二砷诱发自噬可促进细胞调亡。Objective:To investigate the mechanism of arsenic trioxide on apoptosis and autophagy in triple negative breast cancer(TNBC)stem cell MDA-MB-231 cell line.Methods:The stem cells cultured in the second generation were taken for 5 and divided into four groups:control group,arsenic trioxide acting group,Rapa combined with arsenic trioxide group and 3-MA combined with arsenic trioxide group.The apoptosis rate,autophagy and protein expression were observed.Results:For 48 h,the total apoptosis was 40%.When 3MA inhibited the autophagic activity,the apoptosis induced by arsenic trioxide decreased by about 50%,and Rapa promoted the arsenic trioxide induced apoptosis increased by about 50%.Compared with the control group,the intracellular fluorescence intensity was higher in arsenic trioxide,Rapa combined with arsenic trioxide,and 3-MA combined with arsenic trioxide.Bax,Beclin-1 and P62 protein expression were higher in 3-MA and arsenic trioxide group and Rapa group,and Bcl-2 protein expression was lower than the group and Rapa with arsenic trioxide group(P<0.05).Conclusion:Arsenic trioxide induced autophagy and cell apoptosis coexist in MDA-MB-231 stem cells and may be regulated by common genes.Arsenic trioxide induced autophagy can promote cell apoptosis.

关 键 词：三阴乳腺癌 三氧化二砷 细胞凋亡 自噬 

分 类 号：R-33[医药卫生] R737.9