一株嗜盐反硝化菌Halomonas alkaliphile L8的脱氮及其产四氢嘧啶研究  

Study on the Denitrification Performance and Tetrahydropyrimidine Production of Halomonas Alkaliphile L8

作　　者：卢静 武丽娜 田凤蓉 王开春 杨杰 祖航天 吕明生 王淑军 LU Jing;WU Li'na;TIAN Fengrong;WANG Kaichun;YANG Jie;ZU Hangtian;LU Mingsheng;WANG Shujun(School of Life Science,Shanxi University,Taiyuan 030006,China;Zhonglan Lianhai Design and Research Institute Co.,Ltd.,Lianyungang 222004,China;Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Ocean University,Lianyungang 225005,China)

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]中蓝连海设计研究院有限公司,江苏连云港222004 [3]江苏海洋大学江苏省海洋生物资源与环境重点实验室,江苏连云港225005

出　　处：《山西大学学报（自然科学版）》2024年第6期1326-1336,共11页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(32201964)。

摘　　要：石油、医药、海水养殖等产业所产生的废水具有高盐高氮的特点。从连云港市赣榆区盐场沉积土壤中分离得到一株耐盐反硝化细菌L8,经鉴定该菌株为革兰氏阴性菌,盐单胞菌属(Halomonas alkaliphila)。L8菌株最适生长温度为37℃,pH=8.0,NaCl质量浓度0.06 g/mL,具有嗜盐性。L8菌在初始250 mg/L硝态氮质量浓度下反应72 h,对硝酸盐氮的去除率达78%以上。L8菌株具有产四氢嘧啶能力,产量达0.54 mg/mL(培养84 h)。L8是一株耐盐反硝化菌株,在高盐污水处理与工业化生产四氢嘧啶领域具有广阔的应用前景。The wastewater produced by petroleum,pharmaceutical,mariculture and other industries has the characteristics of high salt and high nitrogen.A salt tolerant denitrifying bacterium L8 was isolated from the soil of Ganyu Salt Company in Lianyungang.The strain was identified as Halomonas alkaliphila and a gram-negative bacterium.The optimum growth temperature of L8 was 37℃,pH=8.0 and salinity at 0.06 g/mL.The results showed that the removal rate of nitrate nitrogen by L8 was above 78%when the initial mass concentration of nitrate nitrogen was 250 mg/L for 72 h.The L8 strain had the ability to produce tetrahydropyrimidine,and the yield was 0.54 mg/mL(84 hours of culture).The L8 is a salt tolerant denitrifying strain,which has broad application prospects in the field of high salt wastewater treatment and industrial production of tetrahydropyrimidine.

关 键 词：耐盐 反硝化 四氢嘧啶 高盐含氮水处理 

分 类 号：X172[环境科学与工程—环境科学]