Influence of UV-A irradiation on the selected nutrient composition and volatile profiling of whole milk:Safety and quality evaluation  

作　　者：Anjali H.Kurup Ankit Patras Rishipal R.Bansode Brahmaiah Pendyala Ramasamy Ravi Matthew J.Vergne 

机构地区：[1]Food Biosciences and Technology Program,Department of Agricultural and Environmental Sciences,Tennessee State University,Nashville,37209,TN,USA [2]Center for Excellence in Post-Harvest Technologies,North Carolina Research Campus,North Carolina A&T State University,Kannapolis,NC,USA [3]Department of Pharmaceutical Sciences and Department of Chemistry and Biochemistry,Lipscomb University,Nashville,37204,TN,USA

出　　处：《Food Bioscience》2022年第6期482-491,共10页食品生物科学（英文）

基　　金：funded through a grant from the Agriculture and Food Research Initiative Competitive Grants Program(Grant No.2015-69003-23117,2018-38821-27732,2014-2017-1003416)from the U.S.Department of Agriculture,National Institute of Food and Agriculture.

摘　　要：Aflatoxin(AF),a mycotoxin produced by several genera of fungi,is an important concern in milk-based products due to its toxicity and health consequences.The present study evaluates whether UV-A irradiation can preserve the composition of whole milk(WM)while degradaing these toxins.In addition,this study also investigates the expression of p53 proteins which can be correlated with carcinogenocity.UV-A irradiation experiments were conducted using a near collimated beam system operating at 365 nm.AFs at known concentrations were spiked in WM and irradiated at quantifiable UV doses based on the average volumetric intensity.The impact of ultraviolet light(UV-A)irradiation on volatile compounds,certain amino acids,and oxidative products were evaluated.No significant reduction in amino acids was observed except tryptophan(p<0.05).Therefore,tryptophan particularly gets degraded under UV and generates OH−radical.At 838 mJ/cm2 no significant lipid peroxidation was observed,p<0.05.The volatile profiling showed that alcohols,the key contributor of oxidized flavor was not significantly affected by the UV-A irradiation.As the overall volatile composition was comparable between the untreated and UV-A irradiated WM there may not be an adverse impact on the sensory attributes.Western blotting was used to assess the effect of UV-A irradiated WM on protein expression in HepG2 cells.Because the targeted gene p53 was not considerably altered,we can affirm that UV-A irradiated WM may be safe and not cytotoxic.Herein,the substantial breakdown of AF in WM by UV-A as well as no accumulation of toxic components from protein,lipid,and FAA degradation was observed.This study conclusively proves the performance of the UV-A LED system in degrading AF in WM below the levels recommended by Food and Drug Administration without compromising the product's quality or safety.

关 键 词：UV IRRADIATION Whole milk AFLATOXINS Protein VOLATILES 

分 类 号：TS201.4[轻工技术与工程—食品科学]