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作 者:Mina Volić Saša Đurović Darko Micić Ilinka Pećinar Milena Pantić Branko Bugarski Nataša Obradović
机构地区:[1]University of Belgrade,Innovation Center of Faculty of Technology and Metallurgy,Karnegijeva 4,11000,Belgrade,Serbia [2]University of Belgrade,Institute of General and Physical Chemistry,Studentski trg 41,11000,Belgrade,Serbia [3]University of Belgrade,Faculty of Agriculture,Nemanjina 6,11080,Belgrade,Serbia [4]University of Belgrade,Faculty of Technology and Metallurgy,Karnegijeva 4,11000,Belgrade,Serbia
出 处:《Food Bioscience》2022年第6期936-943,共8页食品生物科学(英文)
基 金:supported by the Ministry of Education,Science and Technological Development of the Republic of Serbia(Contract No.451-03-68/2022-14/200287 and 451-03-68/2022-14/200051).
摘 要:The prevention of food contamination by pathogenic microorganisms is a very important factor for food safety and security during storage time. The essential oils have the potential to be used as antimicrobial agents, but their full potential is limited due to the sensitivity and volatility of bioactive compounds. This study aimed to characterize Thymus vulgaris essential oil, free and nano-encapsulated in alginate-whey protein concentrate carriers using the freeze-drying technique. GC/MS analysis was done to identify the oil composition. The investigation of the antimicrobial effect of free and encapsulated oil was performed on Escherichia coli, Staphylococcus aureus, and Candida albicans, by microdilution method. The free thyme oil exhibited antimicrobial activity against all tested microorganisms in the range of 0.71–1.43 mg/ml. Interestingly, encapsulated oil was more effective at lower concentrations (0.625–1.25 mg/ml), as a consequence of a nano-size carrier (0.25 ± 0.5 μm) together with other properties (encapsulation efficiency of 86.04%). TGA pointed out a significant effect of encapsulation, in comparison to free oil, on the stability of thyme oil at temperatures from 25 to 500 °C. The results indicated a high potential of thyme oil powder carriers as natural antimicrobial additives in food products.
关 键 词:Thymus vulgaris FREEZE-DRYING ENCAPSULATION Antimicrobial activity GC/MS analysis
分 类 号:TS201.3[轻工技术与工程—食品科学]
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