Inhibitory effect of calcium phosphate-coated high-affinity liposomes on Staphylococcus aureus and its biofilms  

作　　者：Lin Lin Pin Zhang Changzhu Li Zichun Hua Haiying Cui 

机构地区：[1]School of Food and Biological Engineering,Jiangsu University,Zhenjiang,212013,China [2]State Key Laboratory of Utilization of Woody Oil Resource,Hunan Academy of Forestry,Changsha,410007,China [3]School of Life Sciences,Nanjing University,Nanjing,210023,China [4]Changzhou High-Tech Research Institute of Nanjing University,Changzhou,213164,China

出　　处：《Food Bioscience》2022年第6期954-964,共11页食品生物科学（英文）

基　　金：financially supported by State Key Laboratory of Utilization of Woody Oil Resource(Grant no.2019XK 2002);National Natural Science Foundation of China(Grant no.31972172);Natural Science Foundation of Jiangsu Province(Grant no.BK20201417);Jiangsu Province Research Fund(Grant no.JNHB-131);Jiangsu University Research Fund(Grant no.11JDG050).

摘　　要：The contamination of Staphylococcus aureus(S.aureus)and its biofilm poses a serious challenge to food safety.In this study,a high-affinity liposome coated with calcium phosphate was developed to provide a novel strategy for antibacterial packaging.Litsea cubeba essential oil(LC-EO)-loaded liposomes with various anionic surfactants including sodium deoxycholate(SDC),sodium dodecyl sulfate(SDS),and sodium dodecyl benzene sulfonate(SDBS)were first synthesized using a film-ultrasonic dispersion method,then an attempt was made to coat calcium phosphate(CaP)shell layers on the surface of different anionic liposomes using a sequential deposition method.Anionic liposomes synthesized with the addition of SDBS(SDBS/EO-Ls)were found to be effective precursors for the successful capping of CaP shells.The SDBS/EO-Ls coated with CaP shells(CaP/SDBS/EO-Ls)were then characterized,including physicochemical properties,stability,and antibacterial and anti-biofilm activities.Results showed that the particle size of SDBS/EO-Ls can be increased by about 40 nm after being coated with a CaP shell,and the Zeta potential value can be decreased by about 25 mV.At the same time,the CaP coating helped to prevent the leakage of essential oils during storage.The prepared CaP/SDBS/EO-Ls had a better inhibitory effect against S.aureus and biofilm due to their high affinity.Even when CaP/SDBS/EO-Ls were applied in two different food systems(smoked bean curd and beef),they still had a long-lasting antibacterial effect.The CaP/SDBS/EO-Ls developed in this study could be used to develop a novel antimicrobial packaging additive.

关 键 词：Litsea cubeba essential oil Anionic liposomes Calcium phosphate Staphylococcus aureus ANTIBIOFILM 

分 类 号：TS201.3[轻工技术与工程—食品科学]