Gelatins and antioxidant peptides from Skipjack tuna(Katsuwonus pelamis)skins:Purification,characterization,and cytoprotection on ultraviolet-A injured human skin fibroblasts  

作　　者：Shuang-Yi Zhang Yu-Qin Zhao Yu-Mei Wang Xiu-Rong Yang Chang-Feng Chi Bin Wang 

机构地区：[1]Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products,School of Food and Pharmacy,Zhejiang Ocean University,Zhoushan,316022,China [2]National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources,School of Marine Science and Technology,Zhejiang Ocean University,Zhoushan,316022,China

出　　处：《Food Bioscience》2022年第6期1724-1734,共11页食品生物科学（英文）

基　　金：funded by the National Natural Science Foundation of China(No.82073764);the Ten-thousand Talents Plan of Zhejiang Province(No.2019R52026).

摘　　要：For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1.61%,and 50.97±1.44%,respectively.Due to the high imino acid contents(228,216,and 213 residues/1000 residues for STG-A,STG-E,and STG-H,respectively),STG-A showed the highest transparency and gel strength properties.Amino acid analysis,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),ultraviolet(UV)and Fourier transform infrared spectroscopy(FTIR)analysis confirmed that STG-A,STG-E,and STG-H kept the main structure of type I collagen,but enzyme and hot water extraction methods showed much stronger hydrolysis ability onαandβchains.Moreover,neutrase hydrolysate(STG-AH)of STG-A showed the highest 1,1-diphenyl-2-picrylhydrazyl radical(DPPH•)scavenging activity and the fraction STG-AH-І(<3.5 kDa)from STG-AH prepared by ultrafiltration could significantly protect human skin fibroblasts(HSFBs)against ultraviolet-A(UVA)injury.Furthermore,nineteen peptides with high antioxidant activity were purified from STG-AH-Іand identified.These results suggested that STG-AH and its derived antioxidant peptides could serve as potential ingredients applied in health benefiting products for preventing UVA injury.

关 键 词：Skipjack tuna(Katsuwonus pelamis) Skin gelatin PEPTIDE Antioxidant activity CYTOPROTECTION Ultraviolet-A injury 

分 类 号：O62[理学—有机化学]