Modulation of Keap1-Nrf2-ARE signaling pathway by oxyresveratrol,a derivative of resveratrol from grape skin  

作　　者：Xinqi Ma Yuqiu Li Chengyu Lv Bin Liu Cuiping Yuan Wei Huang Quan Luo Yechen Xiao Chang Sun Tiezhu Li Jie Zhang 

机构地区：[1]College of Food Science and Engineering,Yanbian University,Yanji,133002,China [2]Institute of Agro-food Technology,Jilin Academy of Agricultural Sciences,Changchun,130033,China [3]College of Food Science and Engineering,Jilin University,Changchun,130062,China

出　　处：《Food Bioscience》2022年第6期2130-2137,共8页食品生物科学（英文）

基　　金：supported by the Agricultural Science and Technology Innovation Program of Jilin Province(KYJF2021JQ010,CXGC2021TD006 and CXGC2021RCY028);the Capital Construction Funds within the Provincial Budget in 2022(2022c043-12);the Science and Technology Development Project Foundation of Jilin Province(20210203182SF);the Administration of Traditional Chinese Medicine of Jilin Province(2021100);the National Key Research and Development Program(2021YFD1200103).

摘　　要：Grape skin,one of the by-products of grape processing,is rich in oxyresveratrol and other bioactive compounds.Oxyresveratrol is a polyphenolic non-flavonoid with anti-inflammatory and anti-aging properties among other biological activities,but little research has been done on its antioxidant mechanism.This study aims to evaluate the regulation of oxyresveratrol on Keap1-Nrf2-ARE signaling pathway.Firstly,oxyresveratrol in grape skin was detected by HPLC-MS/MS,and then its interaction mechanism with Keap1 was investigated by molecular docking.The result of Western blot further confirmed that oxyresveratrol can bind with Nrf2 to induce its nuclear translocation.The Luciferase reporter gene analysis revealed the agonistic effects of oxyresveratrol on ARE.Oxyresveratrol increased not only the mRNA expression levels of ARE-mediated genes NQO1,HO-1,and GCLM,but also their protein expression levels,suggesting that oxyresveratrol inhibited H_(2)O_(2)-induced oxidative damage.Therefore,oxyresveratrol can be applied as a potential antioxidant by activating the Keap-Nrf2-ARE signaling pathway.

关 键 词：OXYRESVERATROL ANTIOXIDANT Keap1-Nrf2-ARE Molecular docking 

分 类 号：TS201[轻工技术与工程—食品科学]