Evaluation of antibacterial and toxicological activities of essential oil of Ocimum gratissimum L.and its major constituent eugenol  

作　　者：Julio Cesar Silva Raimundo Luiz Silva Pereira Thiago Sampaio de Freitas Janaína Esmeraldo Rocha Nair Silva Macedo Carla de Fatima Alves Nonato Marina Leite Linhares Daniely Sampaio Arruda Tavares Francisco Assis Bezerra da Cunha Henrique Douglas Melo Coutinho Sidney Gonçalo de Lima Francisco Nascimento Pereira-Junior Francisco Paulo Araújo Maia Ivo Cavalcante Pita Neto Fabiola Fernandes Galvão Rodrigues George Joaquim Garcia Santos 

机构地区：[1]Department of Biological Chemistry,Regional University of Cariri,R.Cel.Antonio Luis 1161,63105000,Crato,CE,Brazil [2]University Center Doutor Leão Sampaio,Av.Leão Sampaio 400,63040000,Juazeiro do Norte,CE,Brazil [3]Research and Development Center for Medicines,Federal University of Ceara,R.Coronel Nunes de Melo 1000,60430275,Fortaleza,CE,Brazil [4]Chemistry Department,Federal University of Piaui,Campus Universitário Ministro Petrônio Portella,64049550,Teresina,PI,Brazil [5]Center for Agricultural and Biodiversity Sciences - CCAB,Federal University of Cariri,Crato,Ceará,Brazil [6]CECAPE - College of Dentistry,Juazeiro do Norte,CE,63024-015,Brazil

出　　处：《Food Bioscience》2022年第6期2152-2159,共8页食品生物科学（英文）

摘　　要：The present study aimed to comparatively analyze the antibacterial activity,combined with antibiotics,and toxicological activity of the essential oil of O.gratissimum L.and its major compound,eugenol.The phytochemical analysis of the essential oil was performed by gas chromatography coupled with mass spectrometry.The minimum inhibitory concentration and combined antibiotic activity were performed by microdilution.Drosophila melanogaster was the model organism chosen to evaluate the toxicological response of substances.The chromatographic assay showed eugenol as the major compound of the essential oil of O.gratissimum L.The microbiological assays showed that in terms of antibacterial activity,both products showed similar activity,quite significant in Gram-negatives,such as those of strains of Pseudomonas aeruginosa.Similar results were verified in the modulatory activity,allowing the verification that the bioactivity performed by the essential oil of O.gratissimum L.is mainly influenced by its major constituent.Although the EC50 of the compounds varied subtly,the essential oil was less toxic compared to eugenol.The information obtained in the present study demonstrates that the essential oil of O.gratissimum L.is a promising antibacterial agent and better tolerated than its majority compound alone,in the model organism D.melanogaster.

关 键 词：Antibacterial agents Drosophila melanogaster EUGENOL Ocimum gratisismumum L.essential oils 

分 类 号：TS201.2[轻工技术与工程—食品科学]