微波辅助水解/酶比色法快速测定特殊医学用途配方食品中胆碱  

Rapid Determination of Choline in Food for Special Medical Purpose by Enzyme Colorimetry Using Microwave-assisted Hydrolysis

作　　者：崔梦琪 罗璇 娄湘琴 梅英杰 牟丹 安迈瑞 李政 张贞理 CUI Mengqi;LUO Xuan;LOU Xiangqin;MEI Yingjie;MOU Dan;AN Mairui;LI Zheng;ZHANG Zhenli(Zibo Municipal General Institute of Inspection and Metrology,Zibo 255000,China;Inspection and Testing Center in Zichuan District of Zibo,Zibo 255000,China)

机构地区：[1]淄博市检验检测计量研究总院,淄博255000 [2]淄博市淄川区检验检测中心,淄博255000

出　　处：《食品与发酵科技》2024年第6期131-136,共6页Food and Fermentation Science & Technology

基　　金：2021年度山东省市场监督管理局科研项目。

摘　　要：该文利用微波辅助水解技术提取特殊医学用途配方食品中胆碱,并采用酶比色法进行测定。胆碱受到胆碱氧化酶的作用释放过氧化氢(H_(2)O_(2)),在过氧化物酶的作用下,与苯酚和4-氨基安替比林反应生成有色物质,利用分光光度法进行外标法定量。对标准曲线、水解试剂、微波辅助加热条件进行了优化,并将确定的最佳方法在不同基质的样品中进行了实验验证。选择1.0 mol/L盐酸(HCl)溶液在100℃下微波辅助水解样品5 min,测定结果和加标回收率与水浴加热水解的国家标准方法相当,微波辅助水解前处理方法简单、快速,准确度高,适用于批量测定特殊医学用途配方食品中胆碱。This paper extracted choline from food for special medical purpose using microwave-assisted hydrolysis,and detected the choline content by enzyme colorimetry.Choline was catalyzed by choline oxidase with subsequent discharge of hydrogen peroxide(H_(2)O_(2)).In the presence of peroxidase,hydrogen peroxide reacted with phenol and 4-aminoantipyrine and colored substances were formed.The external standard method was used for quantitative analysis of choline by spectrophotometry.Standard curve,the hydrolysis-solvent and microwave-assisted hydrolysis heating condition were optimized.The determined optimum method was experimentally verified in the samples of different matrices.Selecting samples with 1.0 mol/L HCl solution for microwave-assisted hydrolysis at 100℃for 5 min,the analysis results and spiked recovery rates agreed quite well with those obtained by the national standard method for water bath heating hydrolysis.The pretreatment method of microwave-assisted hydrolysis is simple,rapid and highly accurate,and it is applicable for batch detection of choline in food for special medical purpose.

关 键 词：微波辅助水解 酶比色法 胆碱 特殊医学用途配方食品 

分 类 号：TS207.3[轻工技术与工程—食品科学]