底板反应蛋白在宫颈癌组织中的表达及其对宫颈癌Hela细胞生物学行为的影响  

Expression of plate-reactive protein in cervical cancer and its effect on biological behavior of cervical cancer Hela cells

作　　者：冀瑞晴 魏敏 王思梦 Ji Ruiqing;Wei Min;Wang Simeng(The First Clinical Medical College of Xuzhou Medical University,Xuzhou 221002,China;Department of Obstetrics and Gynecology,Xuzhou Medical University Affiliated Hospital,Xuzhou 221002,China)

机构地区：[1]徐州医科大学第一临床医学院,徐州221002 [2]徐州医科大学附属医院妇产科,徐州221002

出　　处：《中国综合临床》2024年第6期415-422,共8页Clinical Medicine of China

摘　　要：目的探讨底板反应蛋白(F-spondin, SPON1)在宫颈癌组织中的表达情况及对Hela细胞株增殖、凋亡、侵袭和迁移等生物学行为的影响。方法选取徐州医科大学病理科2022年1月至2023年5月存放的宫颈癌组织和宫颈炎组织标本各80例。免疫组化检测宫颈癌及慢性宫颈炎组织中SPON1的表达情况, 收集对应的临床病理学数据整理分析;用特异小干扰RNA(small disturbance RNA, siRNA)干扰Hela细胞株中SPON1表达, 设置没有转染的细胞为正常对照组(Ctrl组)、阴性对照组(NC siRNA组)和底板反应蛋白敲除组(SPON1 siRNA组), qPCR和蛋白质印迹(Western blot)检测干扰效果, 并采用细胞增殖实验(cell counting kit-8, CCK-8)、流式细胞学技术、Transwell以及划痕实验检测各组细胞增殖、凋亡、侵袭、迁移情况, 每项实验重复3次。符合正态分布的计量资料以xˉ±s表示;3组间均数比较采用单因素方差分析;两两比较使用LSD-t检验。定性资料计算百分率, 组间率的比较采用χ^(2)检验;P<0.05为差异有统计学意义。结果宫颈癌组与慢性宫颈炎组比较, 宫颈癌组织中SPON1的阳性表达率[72.5%(58/80)]高于慢性宫颈炎组织[20.0%(16/80)], 差异具有统计学意义(χ^(2)=44.35, P<0.001);SPON1的阳性表达率与宫颈癌的临床分期、病理分化程度、肿瘤直径大小有关(χ^(2)值分别为4.10、4.98、4.40, P值分别为0.043、0.026、0.036), 与患者的年龄、组织类型无关(χ^(2)值分别为0.72、0.14, P值分别为0.386、0.713)。NC siRNA组、Ctrl组、SPON1 siRNA组、SPON1 mRNA相对表达量分别为1.000±0.014、0.966±0.082、0.365±0.036, SPON1蛋白表达量分别为1.000±0.013、1.022±0.031、0.655±0.026, 3组细胞穿模细胞数分别为(113.3±4.1)、(107.0±3.1)、(80.3±3.2)个, 3组细胞划痕愈合率分别为(56.00±3.45)%、(55.00±5.03)%、(35.33±3.76)%, 3组细胞凋亡率分别为(5.88±0.44)%、(6.27±0.38)%、(10.50±0.39)%。与Ctrl组和NC siRNA组细胞�ObjectiveTo investigate the expression of F-spondin(SPON1)in cervical cancer tissues and its effects on the biological behavior of Hela cell line,including proliferation,apoptosis,invasion,and migration.MethodSelect 80 samples of cervical cancer tissue and 80 samples of cervical inflammation tissue stored in the Pathology Department of Xuzhou Medical University from January 2022 to May 2023.Immunohistochemistry was used to detect the expression of SPON1 in cervical cancer and chronic cervicitis tissues,and the corresponding clinicopathological data were collected for analysis.Small disturbance RNA(siRNA)was used to interfere with the expression of SPON1 in Hela cell lines.Untransfected cells were set as normal control group(Ctrl group),negative control group(NC siRNA group),and siRNA knockout group(SPON1 siRNA group).The interference effect was detected by qPCR and Western blot.Cell proliferation assay(CCK-8),flow cytometry,Transwell,and scratch assay were used to detect the proliferation,apoptosis,invasion,and migration of each group of cells.Each experiment was repeated three times.Quantitative data that conforms to a normal distribution are represented by x¯±s.The comparison of means among the three groups was conducted using one-way analysis of variance;Compare pairwise using LSD-t test.Calculate the percentage based on qualitative data,and compare the rates between groups using the chi square test.P<0.05 indicates a statistically significant difference.ResultsCompared with the chronic cervicitis group,the positive expression rate of SPON1 in cervical cancer tissues(72.5%(58/80))was higher than that in chronic cervicitis tissues(20.0%(16/80)),and the difference was statistically significant(χ^(2)=44.35,P<0.001).The positive expression rate of SPON1 was positively correlated with the clinical stage,pathological differentiation and tumor diameter of cervical cancer(χ^(2) values were 4.10,4.98,and 4.40,respectively;P values were 0.043,0.026,and 0.036,respectively),and were not related to the age and tissue t

关 键 词：宫颈癌 底板反应蛋白 HELA细胞 肿瘤微环境 

分 类 号：R737.33[医药卫生—肿瘤]