COPD大鼠小肠黏膜屏障功能的改变及机制研究  

Changes and Mechanisms of Intestinal Mucosal Barrier Function in Chronicobstructive Pulmonary Disease Rats

作　　者：梁利娜 黎敏 丁瑞峰[1] 王乐乐[1] LIANG Lina;LI Min;DING Ruifeng;WANG Lele(First Affiliated Hospital of Baotou Medical College,Baotou 014010 China)

机构地区：[1]内蒙古科技大学包头医学院第一附属医院,内蒙古包头014010

出　　处：《内蒙古医学杂志》2024年第11期1281-1283,1289,F0002,共5页Inner Mongolia Medical Journal

摘　　要：目的本研究旨在探讨慢性阻塞性肺疾病(COPD)大鼠小肠黏膜屏障功能的改变,以及其可能的影响因素。方法将16只大鼠随机分为对照组和COPD组,每组8只。以香烟烟雾刺激和脂多糖(LPS)气道注入复制COPD大鼠模型,造模成功后取大鼠末端回肠黏膜组织,透射电镜下观察大鼠小肠黏膜杯状细胞和肠上皮细胞数量及形态学改变,评价小肠黏膜屏障损伤情况;采用ELISA法检测大鼠肠道黏膜黏蛋白2(MUC2)及白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的表达;采用qPCR检测两组大鼠肠道黏膜MUC2 mRNA的表达。结果经透射电镜观察,COPD组大鼠小肠肠上皮细胞排列紊乱,细胞核及内质网形态模糊、结构不清晰,细胞间桥粒连接模糊。COPD组大鼠小肠黏膜MUC2、MUC2 mRNA明显降低(P<0.05),促炎细胞因子(TNF-α、IL-1β)明显增高(P<0.05)。结论COPD大鼠存在小肠黏膜屏障受损,其损伤机制可能与MUC2减少及肠道炎症反应增强有关。Objective The purpose of this research was to explore the alterations in intestinal mucosal barrier performance of rats with chronic obstructive pulmonary disease-COPD,and its potential cause.Method 16 rats were randomly split into two groups:the control group(n=8)and the COPD group(n=8).Under transmission electron microscope,the number and morphological changes of goblet cells and intestinal epithelial cells were observed in a COPD rat model induced by cigarette smoke stimulation and intratracheal injection of lipopolysaccharide(LPS).ELISA was used to detect the expression of MUC2,Il-1βand TNF-αin the intestinal mucosa of the rats,while q-PCR was utilized to detect MUC2 mRNA.Results In the COPD group,the intestinal epithelial cells were arranged irregularly,the nuclear shape was irregular,the endoplasmic reticulum structure was unclear,and the desmosome connection between the cells was blurred.The COPD group's mRNA levels of MUC2 and MUC2 in the small intestinal mucosa were significantly reduced(P<0.05)while the Proinflammatory cytokine levels of TNF-αand Il-1βwere significantly augmented(P<0.05).Conclusions This suggests that the intestinal mucosal barrier is impaired in COPD rats,likely due to the decrease of MUC2 and the augmentation of intestinal inflammatory reaction.

关 键 词：慢性阻塞性肺疾病 肠道黏膜屏障 细胞因子 黏蛋白2 

分 类 号：R574[医药卫生—消化系统]